SRI-011381 (C381)

製品コードS6654 バッチS665403

印刷

化学情報

Synonyms

C381

Storage
(From the date of receipt)

3 years -20°C powder

化学式

C₂₀H₃₁N₃O

分子量

329.48

CAS No.

1629138-41-5

Solubility (25°C)*

体外

DMSO

69 mg/mL (209.42 mM)

Ethanol

69 mg/mL (209.42 mM)

Water

Insoluble

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	3.000mg/ml (9.11mM)	Taking the 1 mL working solution as an example, add 50 μL of 60 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	SRI-011381 (C381) is a novel agonist of the TGF-beta signaling pathway for treatment of Alzheimer's disease.C381 physically targets the lysosome, promotes lysosomal acidification, increases breakdown of lysosomal cargo, and improves lysosome resilience to damage.
in vitro	By partially reversing the effect of sodium ferulate (SF) on the expression of fibrosis-related proteins, SRI-011381 (C381) increases TGF-β1, NALP3, collagen-1, and α-SMA expression.^[2]
in vivo	A small-molecule agonist of TGFβ signaling, SRI-011381 (C381) has no effect on outflow tract (OFT) expansion in zebrafish, suggesting that hyperactivation of TGFβ signaling is not sufficient to induce aneurysm.^[3]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	pulmonary fibroblasts of silicosis mouse model
	濃度	10 µM
	反応時間	--
	実験の流れ	The tissues are extracted in a sterile environment, rinsed in PBS 3 times, and then cut into 1-mm³ tissue blocks. After repeated digestion with 0.25% trypsin at 37 ℃, the histolysate is evenly spread in a 10-cm petri dish. A high-glucose medium containing 10% fetal bovine serum, penicillin, and streptomycin is added to the petri dish. The cells are cultured in an incubator, and the medium is exchanged every 2-3 days. When the cells reached 80-90% confluence, they are passaged according to the ratio of 1: 3, and the fifth passage cells are taken for experimental study. Pulmonary fibroblasts are identified using anti-vimentin antibody with fluorescent secondary antibodies under a fluorescence microscope. For in vitro experiments, a range of concentrations of SF solution (0.04, 0.1, 0.18 and 0.28 mg/mL) are prepared for the treatment of fibroblasts. A 10 μM solution of SRI-011381 (C381) is used for the activation of TGF-β1 signaling.
動物実験	動物モデル	Zebrafish carrying ltbp3^fb28 and ltbp1^fb29
	投薬量	500 µM
	投与方法	exposed

参考

https://pubmed.ncbi.nlm.nih.gov/30914160/
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8214818/
https://pubmed.ncbi.nlm.nih.gov/35098309/

https://pubmed.ncbi.nlm.nih.gov/35259016/

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Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

LINC02679 regulates TRIML2 to promote gastric cancer proliferation and invasion via targeting miR-5004-3p [ Noncoding RNA Res, 2025, 13:1-14]	PubMed: 40276014
Connexin 43 controls metastatic behavior in triple negative breast cancer through TGFβ1-Smad3-intergin αV signaling axis Based on optical image diagnosis [ SLAS Technol, 2024, 29(5):100190]	PubMed: 39299505
Latent TGFβ binding proteins 1 and 3 protect the larval zebrafish outflow tract from aneurysmal dilatation [ Dis Model Mech, 2022, dmm.046979]	PubMed: 35098309
Cartilage Endplate Stem Cells Transdifferentiate Into Nucleus Pulposus Cells via Autocrine Exosomes [ Front Cell Dev Biol, 2021, 9:648201]	PubMed: 33748142
MicroRNA-210-5p alleviates cardiac fibrosis via targeting transforming growth factor-beta type I receptor in rats on high sodium chloride (NaCl)-based diet [ Eur J Pharmacol, 2021, 912:174587]	PubMed: 34678242
MMP11 promotes the proliferation and progression of breast cancer through stabilizing Smad2 protein [ Oncol Rep, 2021, 45(4)16]	PubMed: 33649832

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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