SRI-011381 (C381)

製品コードS6654 バッチS665403

化学情報

	Synonyms	C381	Storage (From the date of receipt)	3 years -20°C powder
	化学式	C₂₀H₃₁N₃O
	分子量	329.48	CAS No.	1629138-41-5
Solubility (25°C)*	体外	DMSO	69 mg/mL (209.42 mM)
		Ethanol	69 mg/mL (209.42 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	SRI-011381 (C381) is a novel agonist of the TGF-beta signaling pathway for treatment of Alzheimer's disease.C381 physically targets the lysosome, promotes lysosomal acidification, increases breakdown of lysosomal cargo, and improves lysosome resilience to damage.
in vitro	SRI-011381 partially reverses the effect of sodium ferulate (SF) on the expression of fibrosis-related proteins, by increasing TGF-β1, NALP3, collagen-1, and α-SMA expression.^[2]
in vivo	SRI-011381, a small-molecule agonist of TGFβ signaling, has no effect on outflow tract (OFT) expansion in zebrafish, suggesting that hyperactivation of TGFβ signaling is not sufficient to induce aneurysm.^[3]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	pulmonary fibroblasts of silicosis mouse model
	濃度	10 µM
	反応時間	--
	実験の流れ	The tissues are extracted in a sterile environment, rinsed in PBS 3 times, and then cut into 1-mm³ tissue blocks. After repeated digestion with 0.25% trypsin at 37 ℃, the histolysate is evenly spread in a 10-cm petri dish. A high-glucose medium containing 10% fetal bovine serum, penicillin, and streptomycin is added to the petri dish. The cells are cultured in an incubator, and the medium is exchanged every 2-3 days. When the cells reached 80-90% confluence, they are passaged according to the ratio of 1: 3, and the fifth passage cells are taken for experimental study. Pulmonary fibroblasts are identified using anti-vimentin antibody with fluorescent secondary antibodies under a fluorescence microscope. For in vitro experiments, a range of concentrations of SF solution (0.04, 0.1, 0.18 and 0.28 mg/mL) are prepared for the treatment of fibroblasts. A 10 μM SRI-011381 solution is used for the activation of TGF-β1 signaling.
動物実験	動物モデル	Zebrafish carrying ltbp3^fb28 and ltbp1^fb29
	投薬量	500 µM
	投与方法	exposed

参考

[1] Hu L,et al.Exp Eye Res. 2019;182:44-56.
[2] Han, Jingyin et al. Medical science monitor : international medical journal of experimental and clinical research vol. 27 e927978. 15 Jun. 2021,
[3] Abrial M, et al. Dis Model Mech. 2022 Mar 1;15(3):dmm046979.

[4] Vest RT, et al. Proc Natl Acad Sci U S A. 2022 Mar 15;119(11):e2121609119.

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Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Latent TGFβ binding proteins 1 and 3 protect the larval zebrafish outflow tract from aneurysmal dilatation [ Dis Model Mech, 2022, dmm.046979]	PubMed: 35098309
Cartilage Endplate Stem Cells Transdifferentiate Into Nucleus Pulposus Cells via Autocrine Exosomes [ Front Cell Dev Biol, 2021, 9:648201]	PubMed: 33748142
MicroRNA-210-5p alleviates cardiac fibrosis via targeting transforming growth factor-beta type I receptor in rats on high sodium chloride (NaCl)-based diet [ Eur J Pharmacol, 2021, 912:174587]	PubMed: 34678242
MMP11 promotes the proliferation and progression of breast cancer through stabilizing Smad2 protein [ Oncol Rep, 2021, 45(4)16]	PubMed: 33649832

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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