SSE15206

製品コードS8789 バッチS878901

化学情報

	Synonyms	N/A	Storage (From the date of receipt)	3 years -20°C powder
	化学式	C₁₉H₂₁N₃O₃S
	分子量	371.45	CAS No.	1370046-40-4
Solubility (25°C)*	体外


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

生物活性

製品説明	SSE15206 is a tubulin polymerization inhibitor that has potent antiproliferative activities in cancer cell lines of different origins and overcomes resistance to microtubule-targeting agents.
in vitro	Treatment of cells with SSE15206 causes aberrant mitosis resulting in G2/M arrest due to incomplete spindle formation, a phenotype often associated with drugs that interfere with microtubule dynamics. This compound inhibits microtubule polymerization both in biochemical and cellular assays by binding to colchicine site in tubulin. Prolonged treatment of cells with this chemical results in apoptotic cell death accompanied by p53 induction^[1].

製品説明

SSE15206 is a tubulin polymerization inhibitor that has potent antiproliferative activities in cancer cell lines of different origins and overcomes resistance to microtubule-targeting agents.

in vitro

Treatment of cells with SSE15206 causes aberrant mitosis resulting in G2/M arrest due to incomplete spindle formation, a phenotype often associated with drugs that interfere with microtubule dynamics. This compound inhibits microtubule polymerization both in biochemical and cellular assays by binding to colchicine site in tubulin. Prolonged treatment of cells with this chemical results in apoptotic cell death accompanied by p53 induction^[1].

プロトコル(参考用のみ)

細胞アッセイ	細胞株	HCT116 cells
	濃度	0.5 μM, 1 μM and 2 μM
	反応時間	4, 8, 12, 24 and 36 hours
	実験の流れ	HCT116 cells are treated with 0.5 μM, 1 μM and 2 μM SSE15206 for 4 and 8 hours. Phosphorylation of histone H3 (S10) and P-MPM2 is determined by western blotting; total-histone H3 and GAPDH are used as loading controls; Or HCT116 cells are treated with 1 μM this compound for 4, 8, 12, 24 and 36 hours and are analyzed through FACS following treatment.

参考

https://www.nature.com/articles/s41598-018-21642-0

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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