Ochromycinone (STA-21)

製品コードS7951 バッチS795102

印刷

化学情報

Chemical Structure Synonyms N/A Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C19H14O4
分子量 306.31 CAS No. 111540-00-2
Solubility (25°C)* 体外 DMSO 21 mg/mL (68.55 mM)
Ethanol 2 mg/mL (6.52 mM)
Water Insoluble
体内 （毎回新しく調製した物を用意してください）
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Ochromycinone (STA-21) is a selective STAT3 inhibitor.
in vitro Ochromycinone (STA-21) inhibits Stat3 DNA binding activity, Stat3 dimerization, and Stat3-dependent luciferase activity in cells. It remarkably inhibits the growth and the survival of the breast carcinoma cells MDA-MB-231, MDA-MB-435s, and MDA-MB-468 that express persistently activated Stat3. [1] In RH30 and RD2 cells, this compound also inhibits cell viability and growth and induced apoptosis through caspases 3, 8 and 9 pathways. [2]
in vivo In IL-1Ra–KO mice, Ochromycinone (STA-21) (0.5 mg/kg, i.p.) reduces the arthritis score and the incidence of arthritis by decreasing the proportion of Th17 cells and increasing the proportion of Treg cells expressing FoxP3. [3]

プロトコル(参考用のみ)

細胞アッセイ 細胞株 MDA-MB-231, MDA-MB-435s, MDA-MB-453, MDA-MB-468, MCF7, and Caov-3 cells
濃度 20 or 30 μM
反応時間 48 h
実験の流れ Human breast cancer cell lines MDA-MB-231, MDA-MB-435s, MDA-MB-453, MDA-MB-468, and MCF7, human ovarian carcinoma cell line Caov-3, and human skin fibroblasts were cultured in DMEM containing 10% FBS and appropriate antibiotics in a 5% CO2 incubator at 37°C. For testing Ochromycinone (STA-21) and other compounds, the cells were exposed to it for 48 h at a final concentration of 20 or 30 μM, respectively. Then, the cells were harvested and subjected to flow-cytometry analysis.
動物実験 動物モデル IL-1Ra–KO mice
投薬量 0.5 mg/kg
投与方法 i.p.

参考

  • https://pubmed.ncbi.nlm.nih.gov/15781862/
  • https://pubmed.ncbi.nlm.nih.gov/17598902/
  • https://pubmed.ncbi.nlm.nih.gov/24757144/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Cationic amphiphilic antihistamines inhibit STAT3 via Ca2+-dependent lysosomal H+ efflux [ Cell Rep, 2023, 42(2):112137] PubMed: 36807142
Microglial depletion impairs glial scar formation and aggravates inflammation partly by inhibiting STAT3 phosphorylation in astrocytes after spinal cord injury [ Neural Regen Res, 2023, 18(6):1325-1331] PubMed: 36453419
TTI-101: A competitive inhibitor of STAT3 that spares oxidative phosphorylation and reverses mechanical allodynia in mouse models of neuropathic pain [ Biochem Pharmacol, 2021, 192:114688] PubMed: 34274354
The nuclear gene rpl18 regulates erythroid maturation via JAK2-STAT3 signaling in zebrafish model of Diamond-Blackfan anemia. [ Cell Death Dis, 2020, 19;11(2):135] PubMed: 32075953
Interleukin-6 Derived From Cancer-Associated Fibroblasts Attenuates the p53 Response to Doxorubicin in Prostate Cancer Cells [ Cell Death Discov, 2020, 2;6:42] PubMed: 32528731
Combination of ERK2 and STAT3 Inhibitors Promotes Anticancer Effects on Acute Lymphoblastic Leukemia Cells [ Cancer Genomics Proteomics, 2020, 17(5):517-527] PubMed: 32859630
JAK/STAT3 and NF-κB Signaling Pathways Regulate Cancer Stem-Cell Properties in Anaplastic Thyroid Cancer Cells. [ Thyroid, 2019, 29(5):674-682] PubMed: 30784360
STAT3 regulates the onset of oxidant-induced senescence in lung fibroblasts [Waters DW Am J Respir Cell Mol Biol, 2019, 10.1165/rcmb.2018-0328OC] PubMed: 30608861

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。