SR1 (StemRegenin 1)

製品コードS2858 バッチS285808

印刷

化学情報

Synonyms

N/A

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₂₄H₂₃N₅OS

分子量

429.54

CAS No.

1227633-49-9

Solubility (25°C)*

体外

DMSO

86 mg/mL (200.21 mM)

Water

Insoluble

Ethanol

Insoluble

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension

CMC-NA

≥5mg/ml

Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	SR1 (StemRegenin 1) is an aryl hydrocarbon receptor (AhR) inhibitor with IC50 of 127 nM in a cell-free assay.
in vitro	StemRegenin 1 (SR1) increases the number of CD34+ cells after 5 to 7 days with an EC50 of 120 nM. Culture of mPB CD34+ cells with cytokines plus this compound (1 μM) for 7 days increases the number of CD34+, CD133+, and CD90+ hematopoietic stem and progenitor cell populations 2.6-, 2.3-, and 10-fold, respectively compared to control cells. Continued culture with it (1 μM) for 3 weeks leads to an 11-fold increase in total nucleated cells (TNC), a 73-fold increase in CD34+ cells as compared to control cultures, and a 1118-fold increase in CD34+ cells relative to input cells. Culture of 1×10³ cord blood CD34+ cells for 5 weeks with SR1 (1 μM) results in the production of 1.69×10⁶ colony forming cells. It induces CD34+ cell expansion by binding and antagonizing AhR as evident by decreased CYP1B1 and AHRR mRNA levels. ^[1] Treatment with this compound (1 μM) accelerates the proliferation of CD34+ cells and decreased the expression levels of VentX in human CD34+ cells. Ectopic expression of VentX prevents SR1-induced expansion of CD34+ cells. ^[2] Sequential coculture with bone morphogenetic protein 4 (20 ng/mL), PGE2 (2 μM), and it (0.75 μM) lead to robust Macaca nemestrina iPSC hematopoietic progenitor cell formation. CD34(+)CD38(-)Thy1(+)CD45RA(-)CD49f(+) cells isolated on the basis of CD34 expression and cultured in SR1 (0.75 μM) expands 3-fold and maintained this long-term repopulating HSC phenotype. ^[3]
in vivo	StemRegenin 1 (SR1) promotes expansion of CB CD34+ cells that contribute to both early and sustained engraftment NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) mice. ^[1]

製品説明

SR1 (StemRegenin 1) is an aryl hydrocarbon receptor (AhR) inhibitor with IC50 of 127 nM in a cell-free assay.

in vitro

StemRegenin 1 (SR1) increases the number of CD34+ cells after 5 to 7 days with an EC50 of 120 nM. Culture of mPB CD34+ cells with cytokines plus this compound (1 μM) for 7 days increases the number of CD34+, CD133+, and CD90+ hematopoietic stem and progenitor cell populations 2.6-, 2.3-, and 10-fold, respectively compared to control cells. Continued culture with it (1 μM) for 3 weeks leads to an 11-fold increase in total nucleated cells (TNC), a 73-fold increase in CD34+ cells as compared to control cultures, and a 1118-fold increase in CD34+ cells relative to input cells. Culture of 1×10³ cord blood CD34+ cells for 5 weeks with SR1 (1 μM) results in the production of 1.69×10⁶ colony forming cells. It induces CD34+ cell expansion by binding and antagonizing AhR as evident by decreased CYP1B1 and AHRR mRNA levels. ^[1]

Treatment with this compound (1 μM) accelerates the proliferation of CD34+ cells and decreased the expression levels of VentX in human CD34+ cells. Ectopic expression of VentX prevents SR1-induced expansion of CD34+ cells. ^[2]

Sequential coculture with bone morphogenetic protein 4 (20 ng/mL), PGE2 (2 μM), and it (0.75 μM) lead to robust Macaca nemestrina iPSC hematopoietic progenitor cell formation. CD34(+)CD38(-)Thy1(+)CD45RA(-)CD49f(+) cells isolated on the basis of CD34 expression and cultured in SR1 (0.75 μM) expands 3-fold and maintained this long-term repopulating HSC phenotype. ^[3]

in vivo

StemRegenin 1 (SR1) promotes expansion of CB CD34+ cells that contribute to both early and sustained engraftment NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) mice. ^[1]

プロトコル(参考用のみ)

キナーゼアッセイ	AhR ligand binding assay
キナーゼアッセイ	SR1 (StemRegenin 1) ligand-treated lysates are incubated at room temperature for 20 min and then photolyzed at 8 cm with 402 nm UV light. Mouse livers are homogenized in buffer (25 mM MOPS, 2 mM EDTA, 0.02% NaN3, and 10% glycerol, pH 7.4) containing 20 mM sodium molybdate and protease inhibitors and centrifuged at 100,000g for 1 h. Dextran-coated charcoal (1%) is added to the photolyzed samples, which are then centrifuged at 3000g for 10 min to remove free ligand. Labeled samples are resolved using 8% acrylamide-tricine-SDS-PAGE, transferred to PVDF membrane, and visualized using autoradiography. Labeled AHR bands are excised and counted using a γ counter.
細胞アッセイ	細胞株	Cell-free assays
	濃度	127 nM
	反応時間
	実験の流れ

参考

https://pubmed.ncbi.nlm.nih.gov/20688981/
https://pubmed.ncbi.nlm.nih.gov/22791709/
https://pubmed.ncbi.nlm.nih.gov/22898598/

カスタマーフィードバック

: Data from [Data independently produced by , , Stem Cells Transl Med, 2017, 6(3):897-909]

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Disparate leukemia mutations converge on nuclear phase-separated condensates [ Cell, 2025, S0092-8674(25)01149-3]	PubMed: 41192422
TET2 deficiency increases the competitive advantage of hematopoietic stem and progenitor cells through upregulation of thrombopoietin receptor signaling [ Nat Commun, 2025, 16(1):2384]	PubMed: 40064887
Enhanced regenerative and developmental potential of embryonal and stem cell-derived platelets compared to adult platelets [ Cell Rep Med, 2025, 6(8):102297]	PubMed: 40795844
L-Kynurenine activates the AHR-PCSK9 pathway to mediate the lipid metabolic and ovarian dysfunction in polycystic ovary syndrome [ Metabolism, 2025, 168:156238]	PubMed: 40169085
Exploring the correlation and mechanism of natural killer cell cytotoxic sensitivity against gastric cancer [ Oncol Res, 2025, 33(6):1485-1494]	PubMed: 40486876
MDS-associated SF3B1 mutations promote aberrant fate choice of hematopoietic stem cell via mis-splicing of mediator kinase module component CDK8 [ bioRxiv, 2025, 2025.08.14.670174]	PubMed: 40894548
Long-term engrafting multilineage hematopoietic cells differentiated from human induced pluripotent stem cells [ Nat Biotechnol, 2024, 10.1038/s41587-024-02360-7]	PubMed: 39223325
NUP98 fusion proteins and KMT2A-MENIN antagonize PRC1.1 to drive gene expression in AML [ Cell Rep, 2024, 43(11):114901]	PubMed: 39475509
TDO2 promotes bladder cancer progression via AhR-mediated SPARC/FILIP1L signaling [ Biochem Pharmacol, 2024, 223:116172]	PubMed: 38552852
StemRegenin 1 attenuates the RANKL-induced osteoclastogenesis via inhibiting AhR-c-src-NF-κB/p-ERK MAPK-NFATc1 signaling pathway [ iScience, 2024, 27(5):109682]	PubMed: 38660403

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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