Sulforaphane

製品コードS5771 バッチS577101

印刷

化学情報

Chemical Structure Synonyms N/A Storage
(From the date of receipt)		 2 years -20°C(in the dark) liquid
化学式

C6H11NOS2
分子量 177.29 CAS No. 4478-93-7
Solubility (25°C)* 体外  
 
 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

生物活性

製品説明 Sulforaphaneは、ブロッコリー、キャベツ、ケールなどのアブラナ科野菜の摂取に由来する天然のイソチオシアネートです。これはNrf2の誘導剤です。Sulforaphaneはまた、ヒストンデアセチラーゼ（HDAC）およびNF-κBの阻害剤でもあります。Sulforaphaneはヘムオキシゲナーゼ-1（HO-1）を増加させ、活性酸素種（ROS）のレベルを減少させます。Sulforaphaneは細胞周期停止とアポトーシスを誘導します。
in vitro

Sulforaphane, reactive oxygen species (ROS) inhibitor, decrease the autophagy induced by CC90003, while cell death strengthens, confirming that this compound induces autophagy by activating ROS/p53.
in vivo

Natural isothiocyanate and potent NRF2 activator sulforaphane reverses diet-induced obesity through a predominantly, but not exclusively, NRF2-dependent mechanism that requires a functional leptin receptor signaling and hyperleptinemia.
密度 1.17 g/mL

プロトコル(参考用のみ)

細胞アッセイ 細胞株 Human CRC cell HCT-116 and SW620
濃度 20 μM
反応時間 24 h
実験の流れ

Cells are cultured in a 96-well microplate at a density of 5×103 cells/well for 24 h. Then, the cells are divided into several groups and treated with different conditions. Cell viability is assessed with CCK-8 after treating drugs. The absorbance value at 450 nm is read with a 96-well plate reader. Nuclear staining with Hoechst33342 is used to analyze the apoptosis. For colony formation assays, an equal number of cells after different treatments are planted in six-well plates.Colonies are visualized by crystal violet staining 14 days after plating.
動物実験 動物モデル diet-induced obese mice
投薬量 25 μl
投与方法 i.p.

参考

  • https://pubmed.ncbi.nlm.nih.gov/30355942/
  • https://pubmed.ncbi.nlm.nih.gov/25936432/
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8643355/
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8947770/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Disruption of the KLHL37-N-Myc complex restores N-Myc degradation and arrests neuroblastoma growth in mouse models [ J Clin Invest, 2025, 135(14)e176655] PubMed: 40493396
Hydroxytyrosol induced ferroptosis through Nrf2 signaling pathway in colorectal cancer cells [ Sci Rep, 2025, 15(1):21271] PubMed: 40594119
Sulforaphane induces cell morphology change and cell apoptosis by activating endoplasmic reticulum stress in glioblastoma [ BMC Cancer, 2025, 25(1):1050] PubMed: 40597933
NFE2L2 protects against Sorafenib-induced Ferroptosis and cardiotoxicity by activating the HO1/ferritin pathway [ Toxicol Appl Pharmacol, 2025, 504:117537] PubMed: 40885409
TMED4 facilitates regulatory T cell suppressive function via ROS homeostasis in tumor and autoimmune mouse models [ J Clin Invest, 2024, 135(1)e179874] PubMed: 39480507
Engineered Cas9 variants bypass Keap1-mediated degradation in human cells and enhance epigenome editing efficiency [ Nucleic Acids Res, 2024, gkae761] PubMed: 39228373
Meningioma achieves malignancy and erastin-induced ferroptosis resistance through FOXM1-AURKA-NRF2 axis [ Redox Biol, 2024, 72:103137] PubMed: 38642502
A novel effect of sulforaphane on promoting mouse granulosa cells proliferation via the NRF2-TKT pathway [ J Adv Res, 2024, S2090-1232(24)00422-3] PubMed: 39341455
ΔNp63α promotes radioresistance in esophageal squamous cell carcinoma through the PLEC-KEAP1-NRF2 feedback loop [ Cell Death Dis, 2024, 15(11):793] PubMed: 39500864
Druggable redox pathways against Mycobacterium abscessus in cystic fibrosis patient-derived airway organoids [ PLoS Pathog, 2023, 19(8):e1011559] PubMed: 37619220

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。