Sulforaphane

製品コードS5771 バッチS577103

印刷

化学情報

Chemical Structure Synonyms N/A Storage
(From the date of receipt)		 2 years -20°C(in the dark) liquid
化学式

C6H11NOS2
分子量 177.29 CAS No. 4478-93-7
Solubility (25°C)* 体外 Water 50 mg/mL (282.02 mM)
 
 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

生物活性

製品説明 Sulforaphane is a naturally occurring isothiocyanate derived from the consumption of cruciferous vegetables, such as broccoli, cabbage, and kale. It is an inducer of Nrf2. Sulforaphane is also an inhibitor of histone deacetylase (HDAC) and NF-κB. Sulforaphane increases heme oxygenase-1 (HO-1) and reduces the levels of reactive oxygen species (ROS). Sulforaphane induces cell cycle arrest and apoptosis.
in vitro

Sulforaphane, reactive oxygen species (ROS) inhibitor, decrease the autophagy induced by CC90003, while cell death strengthens, confirming that CC90003 induces autophagy by activating ROS/p53.[3]
in vivo

Natural isothiocyanate and potent NRF2 activator sulforaphane reverses diet-induced obesity through a predominantly, but not exclusively, NRF2-dependent mechanism that requires a functional leptin receptor signaling and hyperleptinemia.[4]
密度 1.17 g/mL

プロトコル(参考用のみ)

細胞アッセイ 細胞株 Human CRC cell HCT-116 and SW620
濃度 20 μM
反応時間 24 h
実験の流れ

Cells are cultured in a 96-well microplate at a density of 5×103 cells/well for 24 h. Then, the cells are divided into several groups and treated with different conditions. Cell viability is assessed with CCK-8 after treating drugs. The absorbance value at 450 nm is read with a 96-well plate reader. Nuclear staining with Hoechst33342 is used to analyze the apoptosis. For colony formation assays, an equal number of cells after different treatments are planted in six-well plates.Colonies are visualized by crystal violet staining 14 days after plating.
動物実験 動物モデル diet-induced obese mice
投薬量 25 μl
投与方法 i.p.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Druggable redox pathways against Mycobacterium abscessus in cystic fibrosis patient-derived airway organoids [ PLoS Pathog, 2023, 19(8):e1011559] PubMed: 37619220
Druggable redox pathways against Mycobacterium abscessus in cystic fibrosis patient-derived airway organoids [ PLoS Pathog, 2023, 19(8):e1011559] PubMed: 37619220
Identifying autophagy-related genes as potential targets for immunotherapy in tuberculosis [ Int Immunopharmacol, 2023, 118:109956] PubMed: 36931175
Variable p53/Nrf2 crosstalk contributes to triptolide-induced hepatotoxic process [ Toxicol Lett, 2023, 379:67-75] PubMed: 36990132
SARS-CoV-2 virus NSP14 Impairs NRF2/HMOX1 activation by targeting Sirtuin 1 [ Cell Mol Immunol, 2022, 19(8):872-882] PubMed: 35732914
Sulforaphane reduces obesity by reversing leptin resistance [ Elife, 2022, 11e67368] PubMed: 35323110
Downregulation of IL-8 and IL-10 by the Activation of Ca2+-Activated K+ Channel KCa3.1 in THP-1-Derived M2 Macrophages [ Int J Mol Sci, 2022, 23(15)8603] PubMed: 35955737
A Gene Co-Expression Network-Based Drug Repositioning Approach Identifies Candidates for Treatment of Hepatocellular Carcinoma [ Cancers (Basel), 2022, 14(6)1573] PubMed: 35326724
Cystic fibrosis patient-derived bronchial organoids unveil druggable pathways againstMycobacterium abscessus infection [ bioRxiv, 2022, 10.1101/2022.01.03.474765] PubMed: none
Histone deacetylase 3 inhibition alleviates type 2 diabetes mellitus-induced endothelial dysfunction via Nrf2 [ Cell Commun Signal, 2021, 19(1):35] PubMed: 33736642

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。