Datasheet

生物学的記述

Specificity	SV40 T-antigen Antibody [C12F2] detects transgenic levels of total SV40 Large T Antigen protein.
Background	SV40 large T antigen belongs to the polyomavirus family of early proteins essential for viral replication and cellular transformation. It assembles into hexameric structures with a central channel that encircles DNA, featuring distinct domains including a helicase region for unwinding viral DNA at the origin of replication in an ATP-dependent bidirectional manner, an origin-binding domain for specific attachment to sites I and II, and interaction domains that engage host factors. Nuclear localization directs it to modulate cellular processes where it binds retinoblastoma protein (Rb) to release E2F transcription factors, driving S-phase entry and progression through activation of E2F-dependent genes, while complexing with p53 prevents DNA binding and transcriptional activation of cell cycle arrest and apoptosis pathways. Helicase activity melts the double helix at the replication origin, recruits cellular polymerases for viral genome duplication, and supports late gene transcription by stabilizing TBP-TFIIA complexes and interfering with histone deacetylation via HDAC1 inhibition. Interactions with p300/CBP co-activators and other signaling components alter mRNA levels of transcription factors and regulate phosphatase activity indirectly through small t antigen cooperation, promoting cell proliferation and inhibiting growth suppression by factors like CUL7. In permissive cells, these actions enable efficient viral propagation, whereas in non-permissive cells, persistent binding to Rb and p53 dysregulates checkpoint controls, leading to uncontrolled division and transformation observed in experimental models of tumorigenesis.

Specificity

SV40 T-antigen Antibody [C12F2] detects transgenic levels of total SV40 Large T Antigen protein.

Background

SV40 large T antigen belongs to the polyomavirus family of early proteins essential for viral replication and cellular transformation. It assembles into hexameric structures with a central channel that encircles DNA, featuring distinct domains including a helicase region for unwinding viral DNA at the origin of replication in an ATP-dependent bidirectional manner, an origin-binding domain for specific attachment to sites I and II, and interaction domains that engage host factors. Nuclear localization directs it to modulate cellular processes where it binds retinoblastoma protein (Rb) to release E2F transcription factors, driving S-phase entry and progression through activation of E2F-dependent genes, while complexing with p53 prevents DNA binding and transcriptional activation of cell cycle arrest and apoptosis pathways. Helicase activity melts the double helix at the replication origin, recruits cellular polymerases for viral genome duplication, and supports late gene transcription by stabilizing TBP-TFIIA complexes and interfering with histone deacetylation via HDAC1 inhibition. Interactions with p300/CBP co-activators and other signaling components alter mRNA levels of transcription factors and regulate phosphatase activity indirectly through small t antigen cooperation, promoting cell proliferation and inhibiting growth suppression by factors like CUL7. In permissive cells, these actions enable efficient viral propagation, whereas in non-permissive cells, persistent binding to Rb and p53 dysregulates checkpoint controls, leading to uncontrolled division and transformation observed in experimental models of tumorigenesis.

使用情報

Application

WB, IF

Dilution

WB	IF
1:400-1:2000	1:400-1:2000

Reactivity

Transfected cell line - Simian Virus 40

Source

Mouse Monoclonal Antibody

MW

82 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

プロトコール

References

https://pubmed.ncbi.nlm.nih.gov/2450379/
https://pubmed.ncbi.nlm.nih.gov/22994493/

SV40 T-antigen Antibody [C12F2]

生物学的記述

使用情報

References

Application Data