SW033291

製品コードS7900 バッチS790002

印刷

化学情報

Synonyms

N/A

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₂₁H₂₀N₂OS₃

分子量

412.59

CAS No.

459147-39-8

Solubility (25°C)*

体外

DMSO

83 mg/mL (201.16 mM)

Ethanol (warmed with 50ºC water bath)

5 mg/mL (12.11 mM)

Water

Insoluble

体内（毎回新しく調製した物を用意してください）

Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	4.0mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 80 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5%DMSO 1 Corn oil	10.0mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 200 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	SW033291 is a selective inhibitor of 15-hydroxyprostaglandin dehydrogenase (15-PGDH) with IC50 and K_i of 1.5 nM and 0.1 nM, respectively.
in vitro	In Vaco-503 cells, SW033291 (2.5 μM) decreases cellular 15-PGDH enzyme activity by 85%.^[1]
in vivo	In mice receiving a bone marrow transplant, SW033291 (10 mg/kg, i.p.) promotes hematopoietic recovery. In mouse models of colon and liver injury, SW033291 (10 mg/kg, i.p.) reduces the levels of colitis-associated inflammatory cytokines, protects mice from colitis, and promotes liver regeneration. ^[1]

プロトコル(参考用のみ)

キナーゼアッセイ	Activity assays of recombinant 15-PGDH protein
キナーゼアッセイ	For initial characterization of inhibition of 15-PGDH enzyme activity by SW033291, reactions are assembled with experiment specific concentrations of 15-PGDH enzyme, and experiment specific concentrations of SW033291, plus 150 μM NAD(+) and 25 μM PGE2 in reaction buffer (50 mM Tris-HCl, pH7.5, 0.01% Tween 20). The reaction mix is incubated for 15 min at 25 ºC in an Envision Reader. Enzyme activity is determined by following generation of NADH as assayed by recording fluorescence at Ex/Em=340 nM/485 nM every 30s for 3 minutes, commencing immediately after addition of PGE2. IC50 values are calculated with GraphPad Prism 5 software using the sigmoidal dose-response function and plotted against SW033291 concentration.
細胞アッセイ	細胞株	BMSCs
	濃度	1 μM
	反応時間	5 days
	実験の流れ	Cells were treated with indicated concentration of drug for 5 days.
動物実験	動物モデル	Mice receiving a bone marrow transplant, and Mouse models of colon and liver injury
	投薬量	10 mg/kg twice daily
	投与方法	i.p.

参考

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Divalent metal cations stimulate skeleton interoception for new bone formation in mouse injury models [ Nat Commun, 2022, 13(1):535]	PubMed: 35087048
Skeleton interoception regulates bone and fat metabolism through hypothalamic neuroendocrine NPY [ Elife, 2021, 10e70324]	PubMed: 34468315
3D GelMA ICC Scaffolds Combined with SW033291 for Bone Regeneration by Modulating Macrophage Polarization [ Pharmaceutics, 2021, 13(11)1934]	PubMed: 34834349
Sensory nerves regulate mesenchymal stromal cell lineage commitment by tuning sympathetic tones. [ J Clin Invest, 2020, 131554]	PubMed: 32191640
Prostaglandin E2 mediates sensory nerve regulation of bone homeostasis. [ Nat Commun, 2019, 10(1):181]	PubMed: 30643142

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。