Syringic acid

製品コードS3629 バッチS362901

印刷

化学情報

Chemical Structure Synonyms 3,5-dimethyl ether Gallic Acid, 3,5-dimethoxy-4-hydroxy Benzoic Acid, NSC 2129, SYRA Storage
(From the date of receipt)		 3 years -20°C powder (seal)
化学式

C9H10O5
 
分子量 198.17 CAS No. 530-57-4
Solubility (25°C)* 体外 DMSO 39 mg/mL (196.8 mM)
Ethanol 39 mg/mL (196.8 mM)
Water 1 mg/mL (5.04 mM)
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Syringic acid (NSC 2129, SYRA) is a potential antioxidant used in traditional Chinese medicine and is an emerging nutraceutical. It has potential anti-angiogenic, anti-glycating, anti-hyperglycaemic, neuroprotective, and memory-enhancing properties.
in vitro

Syringic acid ameliorates the expressions of TH, DAT and VMAT2 and also significantly attenuates MPTP/p2 induced increased inflammatory markers expression[1]. SYRA pretreatment obviously inhibits H2O2-induced RGC-5 cell injury and also decreases H2O2-induced ROS production and MDA content. Syringic acid may protect RGC-5 cells against apoptosis induced by H2O2 through the activation of PI3K/Akt signaling pathway[3]. Syringic acid shows a time- and dose-dependent (IC50 = 0.95-1.2 mg/mL) antimitogenic effect against cancer cells with little cytotoxicity on normal fibroblasts (≤20%). SYRA alters cell cycle (S/G2-M or G1/G2-M phases) in a time-dependent manner, induces apoptosis, inhibits DNA-binding activity of NFκB (p ≤ 0.0001), chymotrypsin-like/PGPH (peptidyl-glutamyl peptide-hydrolyzing) (p ≤ 0.0001) and the trypsin-like (p ≤ 0.002) activities of 26S proteasome and angiogenesis. SYRA also differentially sensitizes cancer cells to standard chemotherapies with a marked increase in their sensitivity to camptothecin (500-fold), 5FU (20,000-fold), doxorubicin (210-fold), taxol (3134-fold), vinblastine (1000-fold), vincristine (130-fold) and amsacrine (107-fold) compared to standard drugs alone. SYRA exerts its chemotherapeutic and chemo-sensitizing effects through an array of mechanisms including cell-cycle arrest, apoptosis induction, inhibition of cell proliferation, cell migration, angiogenesis, NFκB DNA-binding and proteasome activities[4].
in vivo

Syringic acid has hepatoprotective effects in various animal models. Oral administration of SYRA (10mg/kg of body weight) is reported partial recovery of learning and memory impairment by amyloid β induced neurotoxicity in mice. SYRA also facilitates protective effects on kidney in renal ischemia-reperfusion injury. Antimicrobial activities of SYRA are also reported against various pathogens. Anticancer effects of SYRA are explored on A549 lung carcinoma cells and is found to show apoptotic effects with an IC50 of 30 μM[1]. Syringic acid attenuates the elevation of glycoprotein components in normal and diabetic rats. Syringic acid may increase insulin secretion of pancreatic β-cells and normalize the plasma glucose level[2].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 SW1116, SW837 and CRL1554 cells
濃度 0-2 mg/mL
反応時間 24-72 h
実験の流れ

Colorectal cancer cell lines(SW1116 and SW837) and normal human fibroblasts (CRL1554) are plated in 96-well microtiter plates at a cell density of 27×103 cells/well. Cells are incubated for 24-72 h in a culture medium containing increasing concentration of SA (0-2 mg/mL)at 37℃ in a non-CO2 incubator. After completion of the treatment period, the media are discarded and 100 mL/well of MTT (5 mg/mL membrane filter sterilized culture medium) is added and the plate is incubated for 4 h at 37℃. MTT solution is aspirated and the purple-colored formazan crystals, thus formed, are dissolved in 200mL/well of DMSO:ethanol (1:1; v/v) for 20 min at ambient temperature to lyse the cells and dissolve the colored crystals formed in viable cells.
動物実験 動物モデル male Wistar rats
投薬量 50 mg/kg
投与方法 oral

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Syringic acid promotes proliferation and migration of Schwann cells via down-regulating miR-451-5p [ Acta Biochim Biophys Sin (Shanghai), 2019, 51(12):1198-1207] PubMed: 31748779

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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