TBHQ

製品コードS4990 バッチS499005

印刷

化学情報

Chemical Structure Synonyms Tert-butylhydroquinone Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C10H14O2
分子量 166.22 CAS No. 1948-33-0
Solubility (25°C)* 体外 DMSO 33 mg/mL (198.53 mM)
Ethanol 33 mg/mL (198.53 mM)
Water 4 mg/mL (24.06 mM)
体内 （毎回新しく調製した物を用意してください）
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Tert-butylhydroquinone (TBHQ) is an antioxidant compound which is used to prevent lipid peroxidation and shows multiple cytoprotective actions. It is an activator of Nrf2.
in vitro Tert-butylhydroquinone (tBHQ) is a metabolite of the chemical compound butylated hydroxyanisole and induces Nrf2 activation and conveys protection against hydrogen peroxide, 6-hydroxydopamine, the pesticidal deltamethrin, and other toxicants. This compound preferentially alters the redox status in the mitochondrial compartment in HeLa cells. HeLa cells treated with this chemical show a preferential oxidation of mitochondrial thioredoxin-2 (Trx2), while cellular glutathione and cytosolic thioredoxin-1 are not affected[1]. In cultured H9c2 cells and primary cardiac myocytes, it stimulates Akt phosphorylation and suppresses oxidant-induced apoptosis[2].
in vivo TBHQ treatment elicits significant cytoprotective actions in different organs under pathological conditions. Systemic or local intra-cerebroventricular treatment with this compound in an ischemic stroke model in rats significantly reduces the infarct size and neurological deficits. Administration of this chemical in rats suppresses renal damage and oxidative stress after ischemia and reperfusion injury. In mice with type 1 diabetes, chronic treatment with this compound significantly reduces the degree of glomerular fibrosis and ameliorates proteinuria. This compound prevents left ventricular dilatation and cardiac dysfunction induced by transverse aortic constriction (TAC), and decreases the prevalence of myocardial apoptosis. The beneficial effects of this chemical are associated with an increase in Akt activation, but not related to activations of Nrf2 or AMP-activated protein kinase. This compound-induced Akt activation is accompanied by increased phosphorylation of Bad, glycogen synthase kinase-3β (GSK-3β) and mammalian target of rapamycin (mTOR)[2].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 HeLa cells
濃度 0-15 μM
反応時間 1 h
実験の流れ

HeLa cells are grown until confluent and loaded with 100 µM DCF in loading medium (DMEM with 1% FBS) for 30 min. Cells are washed and then treated with tBHQ (0-15 μM in DMSO) for 1 h. DCF fluorescence is followed in a fluorometer at 488-nm excitation and 520-nm emission.
動物実験 動物モデル male C57BL/6 mice
投薬量 ratio of 1% (w/w) with the animal food powder
投与方法 oral

参考

  • https://pubmed.ncbi.nlm.nih.gov/20429028/
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4531315/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Simvastatin overcomes the pPCK1-pLDHA-SPRINGlac axis-mediated ferroptosis and chemo-immunotherapy resistance in AKT-hyperactivated intrahepatic cholangiocarcinoma [ Cancer Commun (Lond), 2025, 10.1002/cac2.70036] PubMed: 40443016
Hyperoxia-activated Nrf2 regulates ferroptosis in intestinal epithelial cells and intervenes in inflammatory reaction through COX-2/PGE2/EP2 pathway [ Mol Med, 2025, 31(1):1] PubMed: 39754066
NRF2 nuclear translocation and interaction with DUSP1 regulate the osteogenic differentiation of murine mandibular osteoblasts stimulated with Porphyromonas gingivalis lipopolysaccharide [ J Zhejiang Univ Sci B, 2025, 26(9):881-896] PubMed: 40987556
Inhibition of insulin degrading enzyme suppresses osteoclast hyperactivity via enhancing Nrf2-dependent antioxidant response in glucocorticoid-induced osteonecrosis of the femoral head [ Mol Med, 2024, 30(1):111] PubMed: 39085816
BATF is involved in the malignant phenotype and epithelial-mesenchymal transition of colon cancer cells via ERK/PD-L1 signaling [ Histol Histopathol, 2024, 27:18823] PubMed: 39439410
DDRGK1 Enhances Osteosarcoma Chemoresistance via Inhibiting KEAP1-Mediated NRF2 Ubiquitination [ Adv Sci (Weinh), 2023, 10(14):e2204438] PubMed: 36965071
STING Suppresses Mitochondrial VDAC2 to Govern RCC Growth Independent of Innate Immunity [ Adv Sci (Weinh), 2023, 10(3):e2203718] PubMed: 36445063
Fighting cancer by triggering non-canonical mitochondrial permeability transition-driven necrosis through reactive oxygen species induction [ Free Radic Biol Med, 2023, 202:35-45] PubMed: 36963639
NRF2 is a critical regulator and therapeutic target of metal implant particle-incurred bone damage [ Biomaterials, 2022, 288:121742] PubMed: 36030105
AADAC protects colorectal cancer liver colonization from ferroptosis through SLC7A11-dependent inhibition of lipid peroxidation [ J Exp Clin Cancer Res, 2022, 41(1):284] PubMed: 36163032

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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