TC-H 106

製品コードS6738 バッチS673802

化学情報

	Synonyms	Pimelic Diphenylamide 106	Storage (From the date of receipt)	3 years -20°C powder
	化学式	C₂₀H₂₅N₃O₂
	分子量	339.43	CAS No.	937039-45-7
Solubility (25°C)*	体外	DMSO	68 mg/mL (200.33 mM)
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	TC-H 106 (Pimelic Diphenylamide 106) is a slow, tight-binding inhibitor of class I histone deacetylases(HDAC) with Ki value of 148 nM, about 102 nM, 14 nM for HDAC1, HDAC2, HDAC3, respectively.
in vitro	The ability of TC-H 106 to prolong histone acetylation has also been observed in cell culture. Cells treated with TC-H 106 (at a sub-EC10 concentration as determined with an MTS cell proliferation assay) have hyperacetylated histone H3. These cells do not lose significant amounts of acetylated histone H3 even after removal of the inhibitor for hours.^[1].

プロトコル(参考用のみ)

細胞アッセイ	細胞株	lymphoblastoid cell line
	濃度	2 μM
	反応時間	24 h
	実験の流れ	Cells are cultured in RPMI 1640 media with 10% fetal bovine serum and 10 mM HEPES, at 37 °C in 5% CO₂. After the 5th split, the cells are treated with inhibitor TC-H 106 (2 μM) for 24 h, in culture medium. 24 h after treatment, the cells are washed twice with Hanks' balanced salts buffer to remove the inhibitor. A portion of the cell population is then harvested immediately after washing, and referred to as a time 0 point (24 h treatment point), and the rest of the cells are then re-cultured in cell culture media without added inhibitor. The re-cultured cells and controls are then harvested every hour for a total of 7 h.

参考

[1] Chou CJ, et al. J Biol Chem. 2008 19;283(51):35402-9.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

β1 integrin signaling governs necroptosis via the chromatin-remodeling factor CHD4 [ Cell Rep, 2023, 42(11):113322]	PubMed: 37883227
PATJ inhibits histone deacetylase 7 to control tight junction formation and cell polarity [ Cell Mol Life Sci, 2023, 80(11):333]	PubMed: 37878054
Rb-E2F-HDAC Repressor Complexes Control Interferon-Induced Repression of Adenovirus To Promote Persistent Infection [ J Virol, 2022, e0044222]	PubMed: 35546119

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。