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Synonyms | N/A | Storage (From the date of receipt) |
3 years -20°C powder 1 years -80°C in solvent |
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化学式 | C9H2Cl4O2 |
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分子量 | 283.92 | CAS No. | 30675-13-9 | |
Solubility (25°C)* | 体外 | DMSO | 23 mg/mL (81.0 mM) | |
Water | Insoluble | |||
Ethanol | Insoluble | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | TCID is a DUB inhibitor for ubiquitin C-terminal hydrolase L3 with IC50 of 0.6 μM, 125-fold selective to L1. |
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in vitro | TCID is selective for UCH-L3 over UCH-L1 by over 100-fold. [1] NU6027 (10 μM) does not promote YFP-GLT-1 accumulation in intracellular vesicles in transfected MDCK cells, while LDN-57444 (10 μM) promotes YFP-GLT-1 accumulation. NU6027 (10 μM) does not produce long-term lysosomal degradation of GLT-1, while LDN-57444 (10 μM) has such effect. [2] TCID (10 μM) diminishes GlyT2 ubiquitination in brainstem and spinal cord primary neurons, which is more pronounced when UCHL1 is inhibited and when cells are exposed to these inhibitors for longer periods. [3] |
特徴 | TCID demonstrates selectivity for UCH-L3 over UCH-L1 by over 100-fold. |
キナーゼアッセイ | Enzyme Inhibition Studies | |
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Various concentrations (100 μM to 50 nM) of TCID dissolved in DMSO are each added to 2 mL of 1 nM UCH-L1 or 50 pM UCH-L3 in UCH reaction buffer and incubated at 25℃ for 30 min. Ubiquitin C-terminal hydrolysis activity is initiated by adding 2 μL of 50 μM ubiquitin-AMC. The reactions are monitored at 25℃ using a Hitachi F4500 fluorescence spectrophotometer. The AMC fluorophore is excited at 380 nm and the rates of release of free AMC are measured by monitoring the increase in fluorescence emission at 460 nm. The initial velocity Vo is measured for each reaction and plotted against TCID concentrations in order to determine the IC50 values using GraFit V.5 software. |
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A single-cell analysis of breast cancer cell lines to study tumour heterogeneity and drug response [ Nat Commun, 2022, 13(1):1700] | PubMed: 35361799 |
Development of potent and selective inhibitors targeting the papain-like protease of SARS-CoV-2 [ Cell Chem Biol, 2021, S2451-9456(21)00213-0] | PubMed: 33979649 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。