Tempol

製品コードS2910 バッチS291002

印刷

化学情報

Chemical Structure Synonyms 4-Hydroxy-TEMPO Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C9H18NO2
分子量 172.24 CAS No. 2226-96-2
Solubility (25°C)* 体外 DMSO 34 mg/mL (197.39 mM)
Water 34 mg/mL (197.39 mM)
Ethanol 34 mg/mL (197.39 mM)
体内 （毎回新しく調製した物を用意してください）
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution
Saline

この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。

 30.000mg/ml (174.18mM) Taking the 1 mL working solution as an example, add 30 mg of this product to 1 ml of physiological saline (0.9% NaCL solution), mix evenly to make it clear, The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Tempol (4-Hydroxy-TEMPO) is a superoxide scavenger that displays neuroprotective, anti-inflammatory and analgesic effects. Phase 2.
in vitro Tempol (4-Hydroxy-TEMPO) is a stable, cell-permeable nitroxide that acts as a free radical scavenger and nitric oxide spin trap. This compound exhibits radio-protective and anti-proliferative properties. It inhibits oxygen-dependent radiation-induced damage. [1] It induces oxidative stress and enhances the levels of p21WAF1 in HL-60 cells in a dose-dependent manner. [2]
in vivo Tempol reduces oxidant stress-mediated renal dysfunction and injury in the rat. [3] This compound prevents cerebral vessel remodeling in hypertensive rats. [4] It lowers blood pressure and sympathetic nerve activity but not vascular O2- in DOCA-salt rats. [5] It restores mitochondrial and cardiac functions in TNFα-induced oxidative stress and reduces cardiac hypertrophy in chronic hypoxic rats. [6]

プロトコル(参考用のみ)

細胞アッセイ 細胞株 HL60
濃度 0.05–1.6 mM
反応時間 96 h
実験の流れ The antiproliferative effect of Tempol is assessed by the MTT assay after 96 h exposure of the cells to the drug. Briefly, 100,000 cells/mL are seeded onto 96-well plates and allowed to grow for 24 h before treatment with different concentrations of this compound (0.05–1.6 mM).
動物実験 動物モデル Male Wistar rats
投薬量 30 mg/kg
投与方法 bolus injection

参考

  • https://pubmed.ncbi.nlm.nih.gov/1654848/
  • https://pubmed.ncbi.nlm.nih.gov/11033415/
  • https://pubmed.ncbi.nlm.nih.gov/10916089/
  • https://pubmed.ncbi.nlm.nih.gov/20600163/
  • https://pubmed.ncbi.nlm.nih.gov/14707156/
  • https://pubmed.ncbi.nlm.nih.gov/17675574/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Absence of Langerhans cells resulted in over-influx of neutrophils and increased bacterial burden in skin wounds [ Cell Death Dis, 2024, 15(10):760] PubMed: 39424788
NQO1 alleviates renal fibrosis by inhibiting the TLR4/NF-κB and TGF-β/Smad signaling pathways in diabetic nephropathy [ Cell Signal, 2023, 108:110712] PubMed: 37196773
Ginsenoside Rg1 ameliorates chronic intermittent hypoxia-induced vascular endothelial dysfunction by suppressing the formation of mitochondrial reactive oxygen species through the calpain-1 pathway [ J Ginseng Res, 2023, 47(1):144-154] PubMed: 36644390
Impairment of type H vessels by NOX2-mediated endothelial oxidative stress: critical mechanisms and therapeutic targets for bone fragility in streptozotocin-induced type 1 diabetic mice [ Theranostics, 2021, 11(8):3796-3812] PubMed: 33664862
Tempol ameliorates polycystic ovary syndrome through attenuating intestinal oxidative stress and modulating of gut microbiota composition-serum metabolites interaction [ Redox Biol, 2021, 41:101886] PubMed: 33592539
Nedd4 ubiquitylates VDAC2/3 to suppress erastin-induced ferroptosis in melanoma. [ Nat Commun, 2020, 23;11(1):433] PubMed: 31974380
Myeloperoxidase controls bone turnover by suppressing osteoclast differentiation through modulating reactive oxygen species level [ J Bone Miner Res, 2020, 10.1002/jbmr.4215] PubMed: 33289180
Thioredoxin-interacting protein deficiency alleviates phenotypic alterations of podocytes via inhibition of mTOR activation in diabetic nephropathy [Song S J Cell Physiol, 2019, 10.1002/jcp.28317] PubMed: 30746698
Oscillatory Shear Stress Induces Oxidative Stress via TLR4 Activation in Endothelial Cells. [ Mediators Inflamm, 2019, 2019:7162976] PubMed: 31316302
Endoplasmic reticulum stress and autophagy contribute to cadmium-induced cytotoxicity in retinal pigment epithelial cells. [ Toxicol Lett, 2019, 311:105-113] PubMed: 31054874

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。