Tetracycline

製品コードS4490 バッチS449002

化学情報

	Synonyms	TC	Storage (From the date of receipt)	3 years -20°C powder
	化学式	C₂₂H₂₄N₂O₈
	分子量	444.43	CAS No.	60-54-8
Solubility (25°C)*	体外	DMSO	89 mg/mL (200.25 mM)
		Ethanol	11 mg/mL (24.75 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Tetracycline (TC) is a broad-spectrum antibiotic that exhibits activity against a wide range of microorganisms including gram-positive and gram-negative bacteria, chlamydiae, mycoplasmas, rickettsiae, and protozoan parasites.
in vitro	Tetracycline-induced CK2 expression significantly increases the non–small cell lung cancer (NSCLC) invasion in cells expressing BRMS1 wild-type, but not the S30A mutant.^[2]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	H157 BRMS1 knockdown (KD) cells, H157 stable cells
	濃度	1 µg/mL
	反応時間	48 h
	実験の流れ	H157 BRMS1 knockdown (KD) cells were cotransfected with pcDNA3-luciferase and Tet-on CK2α'. Stable clones were selected by Geneticin (400 µg/mL), Blasticidin (5 µg/mL), and Zeocin (150 µg/mL). Myc/His(6)- CK2α' expression was confirmed by immunoblot after treatment with TCN (1 µg/mL) for 48 h. Then, the H157 BRMS1^KD/luciferase/Tet-on CK2α' cells were infected with pBabe-shRNA–resistant FLAG-BRMS1 wild-type, S30A mutant, or empty vector and selected with puromycin (1 µg/mL). Flag-BRMS1 expression was confirmed by Western blot. H157 stable cells were pretreated with or without TCN (1 µg/mL) for 48 h. the inserts were coated with type IV collagen at a concentration of 0.25 mg/ml. The bottom portion of the wells included 1% FBS-media. Cells were allowed to migrate for 6 hours or invade for 22 hours. At the specified time point, inserts were fixed and stained with crystal violet.

参考

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Cyclocytidine hydrochloride inhibits the synthesis of relaxed circular DNA of hepatitis B virus [ PeerJ, 2022, 10:e13719]	PubMed: 35846878

Cyclocytidine hydrochloride inhibits the synthesis of relaxed circular DNA of hepatitis B virus [ PeerJ, 2022, 10:e13719]

PubMed: 35846878

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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