Tetrahydropalmatine hydrochloride

製品コードS3854 バッチS385401

印刷

化学情報

Chemical Structure Synonyms Gindarine hydrochloride, 1-Tetrahydropalmitine HCl Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C21H25NO4.HCl
分子量 391.89 CAS No. 6024-85-7
Solubility (25°C)* 体外 DMSO 12 mg/mL (30.62 mM)
 
 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Tetrahydropalmatine (THP, Gindarine, 1-Tetrahydropalmitine) is an isoquinoline alkaloid found in several different plant species, mainly in the Corydalis genus (Yan Hu Suo), but also in other plants such as Stephania rotunda. It is a potent muscle relaxant.
in vitro Tetrahydropalmatine inhibits the LPSinduced production of COX-2 protein and mRNA in a dose-dependent manner. It suppresses activation of the NF-kB signaling pathway, which results in regulation of IL-8 expression and specifically blocks the ERK1=2 and p38 MAPK signaling pathways[1]. L-THP results in p53 independent apoptosis via down-regulating XIAP protein by inhibiting MDM2 associated with proteasome-dependent pathway and increased sensitivity of EU-4 cells against doxorubicin. L-THP causes activation of caspase and resulted in apoptosis[2]. l-THP possesses a blocking effect on dopamine D1 and D2 receptors and voltage-sensitive Ca2+ channels[3].
in vivo l-THP could attenuate the locomotor-stimulating effects of oxycodone and inhibit the development and expression of oxycodone behavioral sensitization[3].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 THP-1 cells
濃度 0.2, 1, or 2 mM
反応時間 24 h
実験の流れ Cell viability is examined by MTT assay. In brief, THP-1 cells are seeded at a density of 5×104 cells/mL in 96-well plates. Each batch of cells includes a nontreated group as a control. Tetrahydropalmatine (0.2, 1, or 2 mM) and LPS (2 mg/mL) are then added to each well, after which the plates are incubated for 24 hours at 37℃ under 5% CO2. Next, MTT solutions (5 mg/mL) are added to each well, and the samples are then cultured for an additional 4 hours. The supernatant is then discarded, and 100 mL of dimethylsulfoxide is added to each well. Next, the optical density is read at 590 nm.
動物実験 動物モデル Kunming mice
投薬量 6.25, 12.5, and 18.75 mg/kg
投与方法 administered intragastrically

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Tetrahydropalmatine exerts analgesic effects by promoting apoptosis and inhibiting the activation of glial cells in rats with inflammatory pain [ Mol Pain, 2021, 17:17448069211042117] PubMed: 34505815

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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