Thiazolyl Blue

製品コードS6821 バッチS682101

化学情報

	Synonyms	MTT, Tetrazolium bromide, Methylthiazolyldiphenyl-tetrazolium bromide, Methylthialazole Tetrazolium	Storage (From the date of receipt)	3 years -20°C(in the dark) powder
	化学式	C₁₈H₁₆BrN₅S
	分子量	414.32	CAS No.	298-93-1
Solubility (25°C)*	体外	DMSO	21 mg/mL (50.68 mM)
		Ethanol	7 mg/mL (16.89 mM)
		Water	2 mg/mL (4.82 mM)
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Thiazolyl Blue (MTT, Tetrazolium bromide, Methylthiazolyldiphenyl-tetrazolium bromide, Methylthialazole Tetrazolium) is a cell-permeable and positively charged colorimetric agent that is widely used to detect reductive metabolism in cells and to measure cell proliferation, cytotoxicity, and apoptosis. Thiazolyl Blue (MTT) is reduced from yellow color to purple formazan in living cells.
in vitro	1.Preparation of MTT working solution Dissolve MTT by PBS to obtain 5 mg/mL of MTT. 2.Cell proliferation detection ( 96-well plates) 2.1Inoculated cells:Prepared single cell suspension to prepared with culture medium containing 10% FBS and inoculated into 96-well plate with 1000-10000 cells per well, volume of 100 uL per well. 2.2Incubated cells: 37℃. 5% CO2, Incubate for 24-72 h. 2.3Add 10 μL MTT in per well and incubate for 4 h, Discard the supernatant, For suspended cells, need centrifuge firstly. 2.4Add 100 μL DMSO, shock 10 min to dissolve the crystal completely 2.5Monitor the absorbance increase with an absorbance plate reader at OD = 562 nm.

製品説明

Thiazolyl Blue (MTT, Tetrazolium bromide, Methylthiazolyldiphenyl-tetrazolium bromide, Methylthialazole Tetrazolium) is a cell-permeable and positively charged colorimetric agent that is widely used to detect reductive metabolism in cells and to measure cell proliferation, cytotoxicity, and apoptosis. Thiazolyl Blue (MTT) is reduced from yellow color to purple formazan in living cells.

in vitro

1.Preparation of MTT working solution
Dissolve MTT by PBS to obtain 5 mg/mL of MTT.
2.Cell proliferation detection ( 96-well plates)
2.1Inoculated cells:Prepared single cell suspension to prepared with culture medium containing 10% FBS and inoculated into 96-well plate with 1000-10000 cells per well, volume of 100 uL per well.
2.2Incubated cells: 37℃. 5% CO2, Incubate for 24-72 h.
2.3Add 10 μL MTT in per well and incubate for 4 h, Discard the supernatant, For suspended cells, need centrifuge firstly.
2.4Add 100 μL DMSO, shock 10 min to dissolve the crystal completely
2.5Monitor the absorbance increase with an absorbance plate reader at OD = 562 nm.

プロトコル(参考用のみ)

参考

[1] Berridge MV, et al. Biotechnol Annu Rev. 2005;11:127-52.

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長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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