Datasheet

生物学的記述

Specificity	Thymine Dimer Antibody [E15K19] detects endogenous levels of total Thymine Dimer protein.
Background	Thymine dimer (cis-syn cyclobutane thymine dimer, CPD) is the most abundant UV-B-induced DNA photolesion formed exclusively between adjacent thymine bases on the same strand via [2+2] cycloaddition, classified as a mutagenic non-coding lesion within the cyclobutane pyrimidine dimer family. The cis-syn isomer features covalent C5–C6 bonds forming a rigid cyclobutane ring that saturates the 5,6-double bonds of both thymines, eliminating aromaticity, bending the DNA helix by ~30°, unwinding the TT step by ~40°, widening both grooves, pinching the phosphate backbone, weakening 5'-T:A pairing, and inducing anomalous tilt/twist angles localized primarily to the lesion site while preserving overall B-form stacking. Thymine dimers block replicative DNA/RNA polymerases, triggering nucleotide excision repair (NER) via XPC/Rad4 recognition of helical distortion or photolyase direct reversal; translesion synthesis by Pol η inserts AA opposite TT to minimize mutations, but unrepaired lesions cause C→T transitions at dipyrimidine sites characteristic of skin cancers, including melanoma, basal/squamous cell carcinoma. Negative supercoiling enhances dimer formation by facilitating base unstacking.

Specificity

Thymine Dimer Antibody [E15K19] detects endogenous levels of total Thymine Dimer protein.

Background

Thymine dimer (cis-syn cyclobutane thymine dimer, CPD) is the most abundant UV-B-induced DNA photolesion formed exclusively between adjacent thymine bases on the same strand via [2+2] cycloaddition, classified as a mutagenic non-coding lesion within the cyclobutane pyrimidine dimer family. The cis-syn isomer features covalent C5–C6 bonds forming a rigid cyclobutane ring that saturates the 5,6-double bonds of both thymines, eliminating aromaticity, bending the DNA helix by ~30°, unwinding the TT step by ~40°, widening both grooves, pinching the phosphate backbone, weakening 5'-T:A pairing, and inducing anomalous tilt/twist angles localized primarily to the lesion site while preserving overall B-form stacking. Thymine dimers block replicative DNA/RNA polymerases, triggering nucleotide excision repair (NER) via XPC/Rad4 recognition of helical distortion or photolyase direct reversal; translesion synthesis by Pol η inserts AA opposite TT to minimize mutations, but unrepaired lesions cause C→T transitions at dipyrimidine sites characteristic of skin cancers, including melanoma, basal/squamous cell carcinoma. Negative supercoiling enhances dimer formation by facilitating base unstacking.

使用情報

Application

WB, IP, IHC, IF, ELISA

Dilution

Reactivity

All species

Source

Mouse Monoclonal Antibody

MW

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

プロトコール

References

https://pubmed.ncbi.nlm.nih.gov/19006320/
https://pubmed.ncbi.nlm.nih.gov/12456887/

Thymine Dimer Antibody [E15K19]

生物学的記述

使用情報

References

Application Data