Thymoquinone

製品コードS4761 バッチS476101

印刷

化学情報

Chemical Structure Synonyms N/A Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C10H12O2
分子量 164.20 CAS No. 490-91-5
Solubility (25°C)* 体外 DMSO 32 mg/mL (194.88 mM)
 
 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Thymoquinone (TQ) is the bioactive phytochemical constituent of the seeds oil of Nigella sativa with anti-inflammatory and anti-oxidant effects.
in vitro Thymoquinone (TQ) strongly inhibits the production of nitric oxide (NO) and repressed NO synthase (iNOS), tumor necrosis factor (TNF)-α, cyclooxygenase (COX)−2, interleukin (IL)−6, and IL-1β expression in LPS-activated RAW264.7 cells. TQ acts as a radical scavenger and thereby preserves the enzymatic activity of antioxidants like catalase, glutathione peroxidase, and glutathione-S-transferase. TQ abolishes the activity of IRAK1, but not IRAK4, thereby reducing the activity of downstream NF-κB and AP-1[1]. Treatment with TQ significantly decreases cell viability in COLO205 and HCT116 cells in a dose-dependent manner. TQ treatment significantly decreases the level of phosphorylated p65 in the nucleus, which indicates the inhibition of NF-κB activation by TQ treatment. Treatment with TQ also decreases the expression levels of VEGF, c-Myc and Bcl-2[2].
in vivo Treatment of LPS/D-GalN–induced hepatitis and EtOH/HCl–induced gastritis mouse models with TQ significantly ameliorates disease symptoms[1].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 RAW264.7 cells, peritoneal macrophages, and HEK293 cells
濃度 6.25, 12.5, 25 μM
反応時間 24 h
実験の流れ

The cell viability of RAW264.7 cells, peritoneal macrophages, and HEK293 cells during TQ exposure for 24 h is determined by a conventional MTT assay.
動物実験 動物モデル an HCl/EtOH-induced gastritis mouse model (Male C57BL/6 and ICR mice)
投薬量 5 and 25 mg/kg
投与方法 Oral administration

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

PI3K-AKT Pathway Modulation by Thymoquinone Limits Tumor Growth and Glycolytic Metabolism in Colorectal Cancer [ Int J Mol Sci, 2022, 23(4)2305] PubMed: 35216429
Potential food-drug interaction risk of thymoquinone with warfarin [ Chem Biol Interact, 2022, 365:110070] PubMed: 35921950
Potential herb-drug interaction risk of thymoquinone and phenytoin [ Chem Biol Interact, 2022, 353:109801] PubMed: 34998822

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。