受注:045-509-1970 |
技術サポート:[email protected] 平日9:00〜18:00 1営業日以内にご連絡を差し上げます |
Synonyms | N/A | Storage (From the date of receipt) |
3 years -20°C powder 1 years -80°C in solvent |
|
化学式 | C28H47NO4S |
|||
分子量 | 493.74 | CAS No. | 55297-95-5 | |
Solubility (25°C)* | 体外 | DMSO | 98 mg/mL (198.48 mM) | |
Water | 98 mg/mL (198.48 mM) | |||
Ethanol | 98 mg/mL (198.48 mM) | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | Tiamulin is a semisynthetic pleuromutilin antibiotic that binds to the ribosomal peptidyl transferase centre and inhibits protein synthesis. |
---|---|
in vitro | Tiamulin has a remarkable effect on decreasing the activity of CD73 in vitro and dramatically inhibits colony formation of MDA-MB-231 and 4 T1 cells[1]. Tiamulin is located within the peptidyl transferase center (PTC) of the 50S ribosomal subunit with its tricyclic mutilin core positioned in a tight pocket at the A-tRNA binding site. Tiamulin directly inhibits peptide bond formation. It interacts with 23S rRNA and clearly interferes with the correct positioning of both A- and P-site substrates[2]. |
in vivo | Tiamulin inhibits breast tumor growth and metastasis, mainly achieved by affecting the activity but not expression of CD73. The antitumor effect of Tiamulin results in inhibition of metastasis and angiogenesis[1]. |
細胞アッセイ | 細胞株 | MDA-MB-231 or 4 T1 cells |
---|---|---|
濃度 | 0, 6.25, 12.5, 25, 50 or 100 μg/ml | |
反応時間 | 0, 12, 24, 36 or 48 h | |
実験の流れ | MDA-MB-231 or 4 T1 cells are seeded in 96-well plates at 2 × 104 cells per well and incubated overnight before attached. Then, cells are treated without (control group) or with different concentrations of THF (0, 6.25, 12.5, 25, 50 or 100 μg/ml). After 0, 12, 24, 36 or 48 h, cell viability is assessed by using CCK-8 assays. |
|
動物実験 | 動物モデル | Four-week old female BABL/c mice injected subcutaneously with 4 T1 cells |
投薬量 | 80 mg/kg and 160 mg/kg | |
投与方法 | by oral gavage |
|
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。