TLR2-IN-C29

製品コードS6597 バッチS659703

印刷

化学情報

Chemical Structure Synonyms C29 Storage
(From the date of receipt)		 3 years -20°C powder
化学式

C16H15NO4
分子量 285.29 CAS No. 363600-92-4
Solubility (25°C)* 体外 DMSO 57 mg/mL (199.79 mM)
Ethanol 6 mg/mL (21.03 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 TLR2-IN-C29 (C29) is a Toll-like receptor 2 (TLR2) inhibitor that inhibits TLR2/1 and TLR2/6 signaling.
in vitro

TLR2-IN-C29 inhibites TLR2/1 and TLR2/6 signaling induced by synthetic and bacterial TLR2 agonists in human HEK-TLR2 and THP-1 cells, but only TLR2/1 signaling in murine macrophages. TLR2-IN-C29 fails to inhibit signaling induced by other TLR agonists and TNF-α.[2]
in vivo

TLR2-IN-C29 reduces significantly the growth of tumors in Clear cell Renal Cell (RCC) and Head and Neck Squamous Cell Carcinomas (HNSCC) xenograft mice.[3]

プロトコル(参考用のみ)

細胞アッセイ 細胞株 THP-1 cells, HEK-TLR2 cells, murine macrophages
濃度 10 μM-150 μM
反応時間 5 h
実験の流れ

After transfection, cells are allowed to recover for 48 h and treated for 5 h with medium or stimuli in the presence/absence of C29. Cells are lysed in a passive lysis buffer, and firefly luciferase and Renilla luciferase activities are measured using the Dual-Luciferase Reporter Assay System. Cytokine levels in culture supernatants are analyzed by Multiplex beads in the Cytokine Core Laboratory.
動物実験 動物モデル Ectopic model of RCC (nude female mice beearing A498 cells), Ectopic model of HNSCC (nude female mice beearing 786-O cells)
投薬量 50 mg/kg
投与方法 p.o.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

An invasive zone in human liver cancer identified by Stereo-seq promotes hepatocyte-tumor cell crosstalk, local immunosuppression and tumor progression [ Cell Res, 2023, 33(8):585-603] PubMed: 37337030
Dual RNA-Sequencing and Liquid Chromatography-Mass Spectrometry Unveil Specific Insights on the Pathogenicity of Trichophyton mentagrophytes Complex [ J Invest Dermatol, 2023, 143(3):470-479.e6] PubMed: 38295003
Acrylamide induces the activation of BV2 microglial cells through TLR2/4-mediated LRRK2-NFATc2 signaling cascade [ Food Chem Toxicol, 2023, 176:113775] PubMed: 37037409
Acrylamide induces the activation of BV2 microglial cells through TLR2/4-mediated LRRK2-NFATc2 signaling cascade [ Food Chem Toxicol, 2023, 176:113775] PubMed: 37037409
Airway Prevotella promote TLR2-dependent neutrophil activation and rapid clearance of Streptococcus pneumoniae from the lung [ Nat Commun, 2022, 13(1):3321] PubMed: 35680890
Distinct activities of Vδ1+ T-cells upon different cytomegalovirus reactivation status after haematopoietic transplantation [ Immunology, 2022, 167(3):368-383] PubMed: 35795896
Structural characterization and immunomodulatory mechanisms of two novel glucans from Morchella importuna fruiting bodies [ Int J Biol Macromol, 2021, 183:145-157] PubMed: 33878360

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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