Datasheet

生物学的記述

Specificity	Toll-like Receptor 9 Antibody [M15F23] detects endogenous levels of total Toll-like Receptor 9 protein.
Background	Toll-like receptor 9 belongs to the Toll-like receptor family of pattern recognition receptors and functions primarily as an intracellular sensor for microbial DNA. It possesses an extracellular leucine-rich repeat domain for ligand recognition and a cytoplasmic Toll/interleukin-1 receptor domain that recruits adaptor proteins. TLR9 localizes to the endoplasmic reticulum and undergoes proteolytic cleavage in endolysosomal compartments to generate a mature form capable of ligand binding at acidic pH in resting cells. Unmethylated CpG-containing DNA binds directly to the cleaved TLR9 ectodomain, inducing dimerization and recruitment of MyD88 along with TIRAP to form a signaling complex that activates IRAK4 and IRAK1 kinases. Phosphorylated IRAKs associate with TRAF6, which undergoes K63-linked auto-ubiquitination to activate TAK1, leading to IKK complex phosphorylation and subsequent IκBα degradation, thereby enabling NF-κB nuclear translocation and transcription of proinflammatory cytokines including TNF-α, IL-6, IL-12, and type I interferons. TLR9 signaling also engages the MAPK cascade through p38, JNK, and ERK phosphorylation, amplifying AP-1 activity to coordinate innate immune responses in plasmacytoid dendritic cells, macrophages, and B cells. MyD88-independent TRIF-mediated activation contributes to IRF7 phosphorylation and interferon-β production upon prolonged stimulation. Trafficking from ER through Golgi to lysosomes requires UNC93B1 chaperone activity, with endosomal acidification essential for ligand access and signal initiation. Expression predominates in immune cells, with isoforms arising from alternative splicing influencing ligand specificity. Dysregulation through polymorphisms or overexpression links TLR9 to autoimmune diseases such as systemic lupus erythematosus and rheumatoid arthritis, where self-DNA recognition drives chronic inflammation.

Specificity

Toll-like Receptor 9 Antibody [M15F23] detects endogenous levels of total Toll-like Receptor 9 protein.

Background

Toll-like receptor 9 belongs to the Toll-like receptor family of pattern recognition receptors and functions primarily as an intracellular sensor for microbial DNA. It possesses an extracellular leucine-rich repeat domain for ligand recognition and a cytoplasmic Toll/interleukin-1 receptor domain that recruits adaptor proteins. TLR9 localizes to the endoplasmic reticulum and undergoes proteolytic cleavage in endolysosomal compartments to generate a mature form capable of ligand binding at acidic pH in resting cells. Unmethylated CpG-containing DNA binds directly to the cleaved TLR9 ectodomain, inducing dimerization and recruitment of MyD88 along with TIRAP to form a signaling complex that activates IRAK4 and IRAK1 kinases. Phosphorylated IRAKs associate with TRAF6, which undergoes K63-linked auto-ubiquitination to activate TAK1, leading to IKK complex phosphorylation and subsequent IκBα degradation, thereby enabling NF-κB nuclear translocation and transcription of proinflammatory cytokines including TNF-α, IL-6, IL-12, and type I interferons. TLR9 signaling also engages the MAPK cascade through p38, JNK, and ERK phosphorylation, amplifying AP-1 activity to coordinate innate immune responses in plasmacytoid dendritic cells, macrophages, and B cells. MyD88-independent TRIF-mediated activation contributes to IRF7 phosphorylation and interferon-β production upon prolonged stimulation. Trafficking from ER through Golgi to lysosomes requires UNC93B1 chaperone activity, with endosomal acidification essential for ligand access and signal initiation. Expression predominates in immune cells, with isoforms arising from alternative splicing influencing ligand specificity. Dysregulation through polymorphisms or overexpression links TLR9 to autoimmune diseases such as systemic lupus erythematosus and rheumatoid arthritis, where self-DNA recognition drives chronic inflammation.

使用情報

Application

WB, IP, IF, FCM

Dilution

WB	IP	IF	FCM
1:1000	1:50	1:400-1:1600	1:100-1:400

Reactivity

Human

Source

Rabbit Monoclonal Antibody

MW

130 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

プロトコール

References

https://pubmed.ncbi.nlm.nih.gov/14716310/
https://pubmed.ncbi.nlm.nih.gov/15307186/

Application Data

WB

Validated by Selleck

Lane 1: Ramos, Lane 2: Raji