Topotecan (SKF 104864A)

製品コードS9321 バッチS932104

化学情報

	Synonyms	NSC609699,Nogitecan,SKFS 104864A	Storage (From the date of receipt)	3 years -20°C powder
	化学式	C₂₃H₂₃N₃O₅
	分子量	421.45	CAS No.	123948-87-8
Solubility (25°C)*	体外	DMSO	84 mg/mL (199.31 mM)
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Topotecan（NSC609699、Nogitecan、SKFS 104864A）は、DNAトポイソメラーゼを阻害することで作用する卵巣がんに使用される抗悪性腫瘍薬です。
in vitro	Topotecan exhibits inhibition of outgrowth of the highly invasive and migratory leader cells (LC) population, however, topotecan enrichs the LC subset 20% above the untreated control level.
in vivo	Topotecan (TPT), at least in part by inhibiting expression of Fli-1, significantly ameliorates lupus nephritis in NZBWF1 mice as effectively or better than cyclophosphamide (CYC).

プロトコル(参考用のみ)

細胞アッセイ	細胞株	LC-T2A-GFP lines
	濃度	1 μM
	反応時間	72 h
	実験の流れ	LC-T2A-GFP lines are seeded at 1200 cells per well of a 384-well plate, adhered overnight, and treated with chemotherapeutics olaparib, rucaparib, paclitaxel, topotecan, carboplatin, cyclophosphamide, cisplatin, and doxorubicin at empirically determined clinically relevant doses (1 μM). Cells are maintained at 37 ℃ with 5% CO₂ in treatment for 72 h. Four focal areas per replicate well were imaged using 4X magnification on a bright field.
動物実験	動物モデル	NZBWF1 female mice
	投薬量	0.03 mg/kg, 0.1 mg/kg, 0.3mg/kg
	投与方法	i.p.

参考

https://pubmed.ncbi.nlm.nih.gov/29412654/
https://pubmed.ncbi.nlm.nih.gov/34470672/
https://pubmed.ncbi.nlm.nih.gov/33559345/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

In Vitro Stability and Preclinical Safety Evaluation of High-Dose Intravitreal Topotecan in Rabbits: Impact of Dose and Concentration [ Ophthalmol Sci, 2026, 6(1):100963]	PubMed: 41321844
EZH2 inhibition sensitizes MYC-high medulloblastoma cancers to PARP inhibition by regulating NUPR1-mediated DNA repair [ Oncogene, 2025, 44(6):391-405]	PubMed: 39562655
Elacridar Inhibits BCRP Protein Activity in 2D and 3D Cell Culture Models of Ovarian Cancer and Re-Sensitizes Cells to Cytotoxic Drugs [ Int J Mol Sci, 2025, 26(12)5800]	PubMed: 40565261
Characterization of a pleomorphic rhabdomyosarcoma cell line [ Sci Rep, 2025, 15(1):2893]	PubMed: 39843506
Ubiquitin-specific protease 22 controls melanoma metastasis and vulnerability to ferroptosis through targeting SIRT1/PTEN/PI3K signaling [ MedComm (2020), 2024, 5(8):e684]	PubMed: 39135915
Patient-derived rhabdomyosarcoma cells recapitulate the genetic and transcriptomic landscapes of primary tumors [ iScience, 2024, 27(10):110862]	PubMed: 39319271
Identification and targeting of protein tyrosine kinase 7 (PTK7) as an immunotherapy candidate for neuroblastoma [ Cell Rep Med, 2023, 4(6):101091]	PubMed: 37343516
Temozolomide Sensitizes ARID1A-Mutated Cancers to PARP Inhibitors [ Cancer Res, 2023, 83(16):2750-2762]	PubMed: 37306706
Immune suppressive signaling regulated by latent transforming growth factor beta binding protein 1 promotes metastasis in cervical cancer [ Braz J Med Biol Res, 2023, 55:e12206]	PubMed: 36629522
FL118, acting as a 'molecular glue degrader', binds to dephosphorylates and degrades the oncoprotein DDX5 (p68) to control c-Myc, survivin and mutant Kras against colorectal and pancreatic cancer with high efficacy [ Clin Transl Med, 2022, 12(5):e881]	PubMed: 35604033

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。