TRC051384

製品コードS8305 バッチS830501

印刷

化学情報

Synonyms

N/A

Storage
(From the date of receipt)

3 years -20°C(in the dark) powder

化学式

C₂₅H₃₁N₅O₄

分子量

465.54

CAS No.

867164-40-7

Solubility (25°C)*

体外

DMSO

93 mg/mL (199.76 mM)

Ethanol

5 mg/mL (10.74 mM)

Water

Insoluble

体内（毎回新しく調製した物を用意してください）

Clear solution

2%DMSO 1 20%PEG300 2 5%Tween80 3 73%ddH2O

0.9mg/ml

Taking the 1 mL working solution as an example, add 20 μL of 45 mg/ml clarified DMSO stock solution to 200 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 730 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	TRC051384 is an inducer of heat shock protein 70 (HSP70) and potent anti-inflammatory agent.
in vitro	TRC051384 is a compound that induces HSPA1A/B expression and subsequent Hsp70 production via heat shock factor-1 (HSF1) activation^[2]. TRC051384 increases HSP70B mRNA expression in both HeLa and primary mixed neuronal cells. Induction of HSP70 by TRC051384 involves HSF1 activation and results in elevated chaperone and anti-inflammatory activity^[1].
in vivo	Treatment with TRC051384 significantly reduces stroke associated neuronal injury and disability in a rat model of transient ischemic stroke even when administered 8 hours post onset of ischemia. TRC051384 significantly reduces infarct size, brain edema and improves neurological deficit^[1]. TRC051384 delays thrombus formation in WT mice, the tail bleeding time still being unaltered^[2].

プロトコル(参考用のみ)

細胞アッセイ	細胞株	HeLa cell line (CCL-2, ATCC)
	濃度	6.25 and 12.5 μM
	反応時間	4 h
	実験の流れ	Induction of HSP70B mRNA is carried out by treatment of cells with TRC051384 (6.25 and 12.5 μM) for 4 hours duration for HeLa cell line and total RNA is isolated. For all RNA samples, cDNAs are synthesized and expression of HSP70B mRNA along with expression of 18S rRNA is monitored by real-time PCR employing ABI 7000 system.
動物実験	動物モデル	Sprague Dawley male rats
	投薬量	4.5 mg/kg and 9 mg/kg
	投与方法	i.p.

参考

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

HSP70 attenuates compression-induced apoptosis of nucleus pulposus cells by suppressing mitochondrial fission via upregulating the expression of SIRT3 [ Exp Mol Med, 2022, 10.1038/s12276-022-00745-9]	PubMed: 35338257
HSP90 Inhibitor 17-AAG Attenuates Nucleus Pulposus Inflammation and Catabolism Induced by M1-Polarized Macrophages [ Front Cell Dev Biol, 2021, 9:796974]	PubMed: 35059401
Activation of HSP70 impedes tert-butyl hydroperoxide (t-BHP)-induced apoptosis and senescence of human nucleus pulposus stem cells via inhibiting the JNK/c-Jun pathway [ Mol Cell Biochem, 2021, 10.1007/s11010-021-04052-1]	PubMed: 33511552

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。