Triclosan

製品コードS4541 バッチS454101

印刷

化学情報

Chemical Structure Synonyms Irgasan, Cloxifenolum Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C12H7Cl3O2
分子量 289.54 CAS No. 3380-34-5
Solubility (25°C)* 体外 DMSO 57 mg/mL (196.86 mM)
Ethanol 57 mg/mL (196.86 mM)
Water Insoluble
体内 （毎回新しく調製した物を用意してください）
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Triclosan (Irgasan, Cloxifenolum) は、防腐剤として化粧品やトイレタリー石鹸に使用されるジフェニルエーテル誘導体です。いくつかの静菌作用と静真菌作用があります。
in vitro cancer cells with triclosan at the physiologically related concentrations significantly increases the colony number of the cancer cells assessed by tumor formation assay. This compound-treated cells have a mesenchymal-like morphology and decrease the cell-to-cell adhesion. This chemical-treated cells exhibit decreased E-cadherin while significantly up-regulated levels of EMT markers, namely N-cadherin, vimentin, snail and slug. It may potentiate cancer cells survival in detached condition and motility via the process of EMT.
in vivo Triclosan plays a pathologic role in promoting tissue fibrogenesis, leading to liver carcinogenesis. It accelerates both HCC development and its long-term effects on liver damage, accompanied by fibrosis and inflammation in mice.

プロトコル(参考用のみ)

細胞アッセイ 細胞株 CV-1 cells
濃度 10 μM
反応時間 24 h
実験の流れ CV-1 cells are maintained in DMEM supplemented with 10% FBS, seeded in 96-well plates, and transfected with an expression vector containing a specific nuclear receptor and RXR, along with a luciferase reporter containing the appropriate DNA response element. The assay is first validated with known nuclear receptor ligands as positive controls. For the CAR ligand-binding assay, CV-1 cells are transiently transfected with the expression vector containing the Gal4 DNA-binding domain fused with the ligand-binding domain of murine or human CAR and cotransfected with the luciferase reporter plasmid, mh 100-luc. The day after the transfection, the positive compound of each nuclear receptor or this compound is added and the cells are incubated for an additional 24 h. The luciferase activities are measured and normalized by β-gal activity.
動物実験 動物モデル Male C57BL/6 mice
投薬量 15 mg/kg
投与方法 i.p.

参考

  • https://pubmed.ncbi.nlm.nih.gov/25404284/
  • https://pubmed.ncbi.nlm.nih.gov/25329306/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Controlling distinct signaling states in cultured cancer cells provides a new platform for drug discovery [ FASEB J, 2019, 10.1096/fj.201802603RR] PubMed: 31145643
Triclosan treatment decreased the antitumor effect of sorafenib on hepatocellular carcinoma cells [ OncoTargets and Therapy, 2018, 2945–2954] PubMed: 29849464.0
Triclosan treatment decreased the antitumor effect of sorafenib on hepatocellular carcinoma cells. [ Onco Targets Ther, 2018, 11:2945-2954] PubMed: 29849464

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。