Trigonelline Hydrochloride

製品コードS3802 バッチS380202

印刷

化学情報

Chemical Structure Synonyms Nicotinic acid N-methylbetaine Hydrochloride, Coffearine Hydrochloride, Gynesine Hydrochloride Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C7H8NO2.Cl
分子量 173.6 CAS No. 6138-41-6
Solubility (25°C)* 体外 Water 34 mg/mL (195.85 mM)
DMSO 5 mg/mL (28.8 mM)
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Trigonelline (Nicotinic acid N-methylbetaine, Coffearine, Gynesine), a major alkaloid component of fenugreek, has hypoglycemic, hypolipidemic, neuroprotective, antimigraine, sedative, memory-improving, antibacterial, antiviral, and anti-tumor activities, and also has been shown to reduce diabetic auditory neuropathy and platelet aggregation.
in vitro Trigonelline suppresses lipid droplet accumulation in a concentration (75 and 100 μM) dependent manner. Treatment of adipocyte with of Trigonelline down-regulates the peroxisome proliferator-activated receptor (PPAR) and CCAAT element binding protein (C/EBP- ) mRNA expression, which leads to further down regulation of other gene such as adiponectin, adipogenin, leptin, resistin andadipocyte fatty acid binding protein (aP2) as compared with respective control cells on 5th and 10thday of differentiation [2].
in vivo Trigonelline has been shown to reduce diabetic auditory neuropathy and platelet aggregation. In rabbits, the main parameters for the fenugreek extract (i.g.) are as follows: T1/2(Ka)=0.9 h, T1/2(Ke)=2.2 h, V=0.64 L/kg, area under the curve=1.93 mg/min/L. The main parameters for trigonelline (i.v.) are as follows: T1/2α=10.8 min, T1/2β=44.0 min, K21=0.044/min, K10=0.026/min, K12=0.017/min, area under the concentration-time curve=931.0 mg/min/L. Trigonelline has a moderate rate of absorption and high rate of elimination in the rabbit. Trigonelline has beneficial effects in several human diseases, notably diabetes and central nervous system disease. Its properties include hypoglycemic, hypolipidemic, improving diabetic auditory neuropathy, neuroprotective, antimigraine, sedative, antibacterial, antiviral, and anti-tumor activities; it also improves memory retention and inhibits platelet aggregation[1].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 3T3-L1 cell
濃度 25, 50, 75 and 100 μM
反応時間 24 and 48 h
実験の流れ The 3T3-L1 pre-adipocytes cells are seeded in the 96 well at a density of 1 × 105cells/well. The cells are treated with different concentrations (25, 50, 75 and 100 μM) of Trigonelline. It is incubated at the 37◦C in 5% CO2 incubator for 24 and 48 h and then the culture is treated with WST reagent incubated for 2-4 h. The living cells absorb the WST then it is converted into an orange color product. Then, the intensity of color is measured at 450 nmusing spectra count ELISA reader.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Induction of CD4+ regulatory T cells by stimulation with Staphylococcal Enterotoxin C2 through different signaling pathways [ Biomed Pharmacother, 2021, 143:112204] PubMed: 34560552

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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