Liothyronine (Triiodothyronine, T3)

製品コードS5726 バッチS572601

化学情報

	Synonyms	3,3',5-Triiodo-L-thyronine	Storage (From the date of receipt)	3 years -20°C powder
	化学式	C₁₅H₁₂I₃NO₄
	分子量	650.97	CAS No.	6893-02-3
Solubility (25°C)*	体外	DMSO	100 mg/mL (153.61 mM)
		0.1M NAOH	1.56 mg/mL (2.39 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	T3 (Triiodothyronine)は、成長と発達、代謝、体温、心拍数など、体内のさまざまな生理学的プロセスに影響を与える甲状腺ホルモンです。本製品は可溶ですが、DMSO中で凍結すると沈殿しやすくなります。原液にはNaOHを推奨します。
in vitro	T3 (Triiodothyronine) is one ingredient supplemented in the medium for inducing brown adipocytes differentiation.

プロトコル(参考用のみ)

細胞アッセイ	細胞株	Brown preadipocytes
	濃度	2 nM
	反応時間	2 days
	実験の流れ	Brown preadipocytes were isolated from perirenal adipose tissues of Chuanzhong black goats (1 day after birth). The cells were cultured in DMEM/F12 containing 10% fetal bovine serum and 2% penicillin/streptomycin and placed in an incubator (5% CO₂, 37°C). Until 100% confluence, DMEM/F12 was supplemented with 2% FBS containing 5 μg/ml of insulin, 0.5 mM IBMX, and 2 nM T3 (Triiodothyronine) to induce brown adipocytes differentiation. Two days later, the induced medium was changed to a maintenance medium (DMEM/F12 supplemented with 2% FBS, 5 μg/ml of insulin, and 2 nM of this compound). Cells were harvested 8 days after differentiation. L-carnitine was added to the medium throughout the differentiation process and supplemented at 600 μΜ. SiAMPKα (GAAGAGAAATTCAGAACCT)15 were transfected by Lipofectamine 3000. Lipid droplets were stained using Oil Red O solution. After staining, 1 ml isopropanol was used to dissolve the oil red O dye, and the absorbance at 510 nm was detected. (Data sourced from selleck products)

参考

https://pubmed.ncbi.nlm.nih.gov/35838582/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Protocol for in vitro modeling of specification and morphogenesis of early pancreas development using human pluripotent stem cell-based organoid differentiation [ STAR Protoc, 2025, 6(2):103834]	PubMed: 40402748
GW0742 as a Potential TRα and TRβ Antagonist Reduces the Viability and Metabolic Activity of an Adult Granulosa Tumour Cell Line and Simultaneously Upregulates TRβ Expression [ Cancers (Basel), 2024, 16(23)4069]	PubMed: 39682255
CFH Haploinsufficiency and Complement Alterations in Early-Onset Macular Degeneration [ Invest Ophthalmol Vis Sci, 2024, 65(4):43]	PubMed: 38683564
Transcriptomic Analysis Reveals Fibroblast Growth Factor 11 (FGF11) Role in Brown Adipocytes in Thermogenic Regulation of Goats [ Int J Mol Sci, 2023, 24(13)10838]	PubMed: 37446019
LSD1 in beige adipocytes protects cardiomyocytes against oxygen and glucose deprivation [ Iran J Basic Med Sci, 2023, 26(1):30-36]	PubMed: 36594068
Maternal L-carnitine supplementation promotes brown adipose tissue thermogenesis of newborn goats after cold exposure [ FASEB J, 2022, 36(8):e22461]	PubMed: 35838582
RNA-Seq reveals miRNA role in thermogenic regulation in brown adipose tissues of goats [ BMC Genomics, 2022, 23(1):186]	PubMed: 35255830
Depletion of Toll-Like Receptor-9 Attenuates Renal Tubulointerstitial Fibrosis After Ischemia-Reperfusion Injury [ Front Cell Dev Biol, 2021, 9:641527]	PubMed: 33644078

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。