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Synonyms | Liothyronine, 3,3',5-Triiodo-L-thyronine | Storage (From the date of receipt) |
3 years -20°C powder | ||||||||
化学式 | C15H12I3NO4 |
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分子量 | 650.97 | CAS No. | 6893-02-3 | ||||||||
Solubility (25°C)* | 体外 | DMSO | 100 mg/mL (153.61 mM) | ||||||||
0.1M NAOH | 13 mg/mL (19.97 mM) | ||||||||||
Water | Insoluble | ||||||||||
体内 (毎回新しく調製した物を用意してください) |
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* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | T3 (Triiodothyronine) is a thyroid hormone that affects various physiological process in the body, including growth and development, metabolism, body temperature, and heart rate.This product is soluble but easy to precipitate after freezing in DMSO. NaOH is recommended as a stock solution. |
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in vitro | Triiodothyronine is one ingredient supplemented in the medium for inducing brown adipocytes differentiation.[1] |
細胞アッセイ | 細胞株 | Brown preadipocytes |
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濃度 | 2 nM | |
反応時間 | 2 days | |
実験の流れ | Brown preadipocytes were isolated from perirenal adipose tissues of Chuanzhong black goats (1 day after birth). The cells were cultured in DMEM/F12 containing 10% fetal bovine serum and 2% penicillin/streptomycin and placed in an incubator (5% CO2, 37°C). Until 100% confluence, DMEM/F12 was supplemented with 2% FBS containing 5 μg/ml of insulin, 1 μM dexamethasone, 0.5 mM IBMX, 1 μM Rosiglitazone, and 2 nM triiodothyronine to induce brown adipocytes differentiation. Two days later, the induced medium was changed to a maintenance medium (DMEM/F12 supplemented with 2% FBS, 5 μg/ml of insulin, and 2 nM triiodothyronine). Cells were harvested 8 days after differentiation. L-carnitine was added to the medium throughout the differentiation process and supplemented at 600 μΜ. SiAMPKα (GAAGAGAAATTCAGAACCT)15 were transfected by Lipofectamine 3000. Lipid droplets were stained using Oil Red O solution. After staining, 1 ml isopropanol was used to dissolve the oil red O dye, and the absorbance at 510 nm was detected. |
Transcriptomic Analysis Reveals Fibroblast Growth Factor 11 (FGF11) Role in Brown Adipocytes in Thermogenic Regulation of Goats [ Int J Mol Sci, 2023, 24(13)10838] | PubMed: 37446019 |
Transcriptomic Analysis Reveals Fibroblast Growth Factor 11 (FGF11) Role in Brown Adipocytes in Thermogenic Regulation of Goats [ Int J Mol Sci, 2023, 24(13)10838] | PubMed: 37446019 |
LSD1 in beige adipocytes protects cardiomyocytes against oxygen and glucose deprivation [ Iran J Basic Med Sci, 2023, 26(1):30-36] | PubMed: 36594068 |
Maternal L-carnitine supplementation promotes brown adipose tissue thermogenesis of newborn goats after cold exposure [ FASEB J, 2022, 36(8):e22461] | PubMed: 35838582 |
RNA-Seq reveals miRNA role in thermogenic regulation in brown adipose tissues of goats [ BMC Genomics, 2022, 23(1):186] | PubMed: 35255830 |
Depletion of Toll-Like Receptor-9 Attenuates Renal Tubulointerstitial Fibrosis After Ischemia-Reperfusion Injury [ Front Cell Dev Biol, 2021, 9:641527] | PubMed: 33644078 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。