Triolein

製品コードS3590 バッチS359002

印刷

化学情報

Synonyms

N/A

Storage
(From the date of receipt)

2 years -20°C liquid

化学式

C₅₇H₁₀₄O₆

分子量

885.43

CAS No.

122-32-7

Solubility (25°C)*

体外

DMSO

100 mg/mL (112.93 mM)

Ethanol

6 mg/mL (6.77 mM)

Water

Insoluble

体内（毎回新しく調製した物を用意してください）

Clear solution	5% DMSO 1 95% corn oil	5.0mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	2.5mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 50 mg/ml clarified DMSO stock solution to 400 μL PEG300, mix evenly to clarify it; add 50 μL Tween80 to the above system, mix evenly to clarify it; then continue to add 500 μL ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Triolein is an inhibitor of metalloproteinase-1 (MMP-1) and reduces both the expression of IL-6 and ROS generation in irradiated keratinocytes.
in vitro	Triolein is an inhibitor of metalloproteinase-1 (MMP-1) and reduces both the expression of IL-6 and ROS generation in irradiated keratinocytes, which can be proposed as an active agent against skin photodamage.^[1]
密度	0.908 g/mL

プロトコル(参考用のみ)

細胞アッセイ	細胞株	The immortalized human bulge stem cell line Tel‐E6E7, Human dermal fibroblasts
	濃度	20 µM
	反応時間	24 h
	実験の流れ	Tel‐E6E7 cells were seeded on day one at a density of 2000 cells per cm² on 3T3‐swiss cells feeder layer in cFAD medium. On day five the medium was change to DMEM and F12 (1:1) mixture containing 2 mM glutamine, hydrocortisone (0.4 µg/ml), 2 mM penicillin/streptomycin, Epithelial Growth factor (5 ng/ml) and 10 % fetal bovine serum. On day six the cells were irradiated on PBS using a UVM‐57 UVP handheld UV lamp (302 nm Midrange) at sub‐lethal doses of 3.4 or 6.8 mJ/cm². Immediately, PBS was replaced with medium containing triolein 20 µM. 24 hours later mRNA was extracted from cells using Trizol Reagent. Conditioned culture medium was collected and used to replace the medium of dermal fibroblasts cells plated the day before (75000 cells/cm²) in DMEM supplemented with 10% fetal bovine serum and 2 mM glutamine. After 48 hours dermal fibroblasts mRNA was extracted.

参考

[1] Gustavo J Leirós, et al. J Dermatol Sci. 2017 Feb;85(2):124-130.

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。