Trolox

製品コードS3665 バッチS366502

印刷

化学情報

Chemical Structure Synonyms N/A Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C14H18O4
分子量 250.29 CAS No. 53188-07-1
Solubility (25°C)* 体外 DMSO 50 mg/mL (199.76 mM)
Ethanol 50 mg/mL (199.76 mM)
Water Insoluble
体内 （毎回新しく調製した物を用意してください）
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Trolox is a cell-permeable, water-soluble derivative of vitamin E with potent antioxidant properties.
in vitro Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-Carboxylic Acid) is a vitamin E analog and a direct scavenger of peroxyl and alkoxyl radicals. This compound impairs PT4 cell survival, has minor effects on ROS levels and causes wide deregulation of cell cycle genes[1]. It enhances As2O3-mediated apoptosis in APL, myeloma, and breast cancer cells. This chemical inhibits DNA damage formation induced by singlet oxygen in human lymphoblast WTK-1 cells and protects red blood cells during photodynamic treatment[2].
in vivo After cerebral ischemia reperfusion, Trolox prescription increases anti-apoptotic proteins and decreased proapoptotic proteins thus protects neurons of the hippocampus and caused improvement of memory[3].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 NB4, IM9, and AsR2 cells
濃度 100 μM
反応時間 6 days
実験の流れ NB4, IM9, and AsR2 cells are seeded at 1 × 105 cells/mL in 24-well plates. Cells are treated with various concentrations of As2O3 or doxorubicin, alone or in combination with 100 μM trolox for 6 days. Viable cells are counted by trypan blue exclusion on days 1, 3, and 6. All cells are maintained at a density lower than 1 × 106 cells/mL through dilution as required, and media ± treatment is replaced every third day. MCF-7, MDA-231, and T47D are seeded in 24-well plates at a density of 4000 cells/well. The next day, fresh media containing As2O3 ± this compound is added. On the days indicated, cells are fixed in 10% trichloroacetic acid and subsequently stained with sulforhodamine B (SRB). Bound SRB is solubilized in 10 mM unbuffered Tris (tris(hydroxymethyl)aminomethane), and optical density is measured at 570 nm in a microplate reader.

参考

  • https://pubmed.ncbi.nlm.nih.gov/24587218/
  • https://pubmed.ncbi.nlm.nih.gov/15466933/
  • https://pubmed.ncbi.nlm.nih.gov/28485696/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Senescence-associated lysosomal dysfunction impairs cystine deprivation-induced lipid peroxidation and ferroptosis [ Nat Commun, 2025, 16(1):6617] PubMed: 40731111
Targeting pancreatic cancer glutamine dependency confers vulnerability to GPX4-dependent ferroptosis [ Cell Rep Med, 2025, 6(2):101928] PubMed: 39879992
The G-protein coupled receptor OXER1 is a tissue redox sensor essential for intestinal epithelial barrier integrity [ bioRxiv, 2025, 2025.02.05.636712] PubMed: 39974905
Strong Protection by 4-Hydroxyestrone against Erastin-Induced Ferroptotic Cell Death in Estrogen Receptor-Negative Human Breast Cancer Cells: Evidence for Protein Disulfide Isomerase as a Mechanistic Target for Protection [ Biochemistry, 2024, 63(8):984-999] PubMed: 38569593
EP1 activation inhibits doxorubicin-cardiomyocyte ferroptosis via Nrf2 [ Redox Biol, 2023, 65:102825] PubMed: 37531930
Co-inhibition of glutaminolysis and one-carbon metabolism promotes ROS accumulation leading to enhancement of chemotherapeutic efficacy in anaplastic thyroid cancer [ Cell Death Dis, 2023, 14(8):515] PubMed: 37573361
Co-inhibition of glutaminolysis and one-carbon metabolism promotes ROS accumulation leading to enhancement of chemotherapeutic efficacy in anaplastic thyroid cancer [ Cell Death Dis, 2023, 14(8):515] PubMed: 37573361
Decreased IDO1-dependent tryptophan metabolism in aged lung during influenza [ Eur Respir J, 2021, 57(5)2000443] PubMed: 33243840
pH-dependent rearrangement determines the iron-activation and antitumor activity of artemisinins [ Free Radic Biol Med, 2021, 163:234-242] PubMed: 33359684
Decreased IDO1 Dependent Tryptophan Metabolism in Aged Lung during Influenza [ Eur Respir J, 2020, 2000443] PubMed: 33243840

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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