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Synonyms | ICS 205-930 | Storage (From the date of receipt) |
3 years -20°C powder | |
化学式 | C17H20N2O2 |
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分子量 | 284.35 | CAS No. | 89565-68-4 | |
Solubility (25°C)* | 体外 | Water | 56 mg/mL (196.94 mM) | |
DMSO | 10 mg/mL (35.16 mM) | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | Tropisetron (ICS 205-930) is a 5-HT3 receptor antagonist and α7-nicotinic receptor agonist with an IC50 of 70.1 ± 0.9 nM for 5-HT3 receptor. |
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in vitro | Tropisetron inhibits antigen-induced proliferation and IL-2 production in human peripheral T cells. Tropisetron inhibits the signalling pathways that regulate the activation of the transcription factors NFAT, NF-κB and AP-1, which are known to play a critical role in the immune response[2]. |
in vivo | Tropisetron could ameliorate the ischemic brain injury via decreasing myeloperoxidase activity and TNF-α level. Tropisetron elicits anti-inflammatory effects in experimental model of cisplatin-induced nephrotoxicity and these effects were completely 5-HT3-receptor-independent[3]. |
細胞アッセイ | 細胞株 | Jurkat cells |
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濃度 | 10, 25, 50 μg/ml | |
反応時間 | 30 min | |
実験の流れ | Jurkat cells (1 × 106 cells/ml) were stimulated with either TNFα (2 ng/ml) or PMA (20 ng/ml) plus ionomycin (0.5 ug/ml) for p65, JNK, ERK1 + 2, p38 and IκBα proteins in the presence or absence of tropisetron. Cells were then washed with PBS and proteins extracted in 50 μl of lysis buffer containing 0.5% NP-40. Protein concentration was determined by the Bradford assay and 30 μg of proteins were boiled in Laemmli buffer and electrophoresed in 10% SDS/polyacrylamide gels. |
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動物実験 | 動物モデル | Male albino mice |
投薬量 | 3 mg/kg | |
投与方法 | i.p. |
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Inhibiting 5-hydroxytryptamine receptor 3 alleviates pathological changes of a mouse model of Alzheimer's disease [ Neural Regen Res, 2023, 18(9):2019-2028] | PubMed: 36926728 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。