U18666A

製品コードS9669 バッチS966902

化学情報

	Synonyms	U18	Storage (From the date of receipt)	3 years -20°C powder
	化学式	C₂₅H₄₂ClNO₂
	分子量	424.06	CAS No.	3039-71-2
Solubility (25°C)*	体外	DMSO	85 mg/mL (200.44 mM)
		Ethanol	85 mg/mL (200.44 mM)
		Water	43 mg/mL (101.4 mM)
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	U18666A (U18) is an inhibitor of intra-cellular cholesterol transport that inhibits dengue virus entry and replication.
in vitro	Viral particles are trapped in the Lamp-1 positive late endosome/lysosome compartment when cells are treated with U18666A. In addition, viral replication is also affected by U18666A treatment. An additive anti-viral effect is found when C75, a fatty acid synthase inhibitor, is used in combination with U81666A, suggesting the role of cholesterol and fatty acid in DENV.^[1]
in vivo	When U18666A is administered to cats experimentally infected with type I FIPV, the development of FIP may be suppressed compared with the control group. However, the number of animals with FIP is too low to establish anti-viral effect of U18666A in cats. Two of the five control cats administered PBS alone develops FIP. Four of the five cats administered U18666A develops no signs of FIP. One cat that temporarily developed fever, has no other clinical symptoms, and no gross lesion is noted on an autopsy after the end of the experiment. The FIPV gene is detected intermittently in feces and saliva regardless of the development of FIP or administration of U18666A.^[2]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	BHK21 cells
	濃度	6.15 μM
	反応時間	48 h
	実験の流れ	BHK21 cells are seeded with a cell density of 2×10⁴ cells/well into a 96 well plate 1 day before infection. NGC virus is added at a multiplicity of infection (MOI) of 1 in the presence of 2% FBS and is harvested from the cell supernatant 2 days post infection. Viral titer is quantified by plaque assay. For time of addition experiment of U18666A, cells are pretreated for 16 h with the compound, before virus is added to the cells (16 h pretreatment) or cells are pretreated for 16 h and the compound remained throughout the 48 h of infection (throughout). Alternatively, compound is added only during the 1st hour of infection where most of the entry and fusion occurred (1 h entry), or after the 1st hour of infection (after entry) for the remaining of the infection.
動物実験	動物モデル	SPF cats
	投薬量	2.5 mg/kg, 1.25 mg/kg
	投与方法	SC

参考

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Lipophagy-mediated cholesterol synthesis inhibition is required for the survival of hepatocellular carcinoma under glutamine deprivation [ Redox Biol, 2023, 63:102732]	PubMed: 37150151

Lipophagy-mediated cholesterol synthesis inhibition is required for the survival of hepatocellular carcinoma under glutamine deprivation [ Redox Biol, 2023, 63:102732]

PubMed: 37150151

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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