Datasheet

生物学的記述

Specificity	uPA Antibody [F9E24] detects endogenous levels of total uPA protein.
Background	Urokinase-type plasminogen activator (uPA, PLAU) is a 411-residue secreted serine protease of the chymotrypsin family essential for pericellular proteolysis in the plasminogen activation system. Synthesized as a single-chain proenzyme (scuPA, 52 kDa) with an N-terminal growth factor domain (GFD) for uPAR binding, a kringle domain (KD) mediating fibrin and plasminogen interactions, a connecting peptide, and a catalytic serine protease domain (SPD) containing the His204-Asp256-Ser344 triad, uPA is activated by plasmin cleavage to a two-chain form (tc-uPA). By binding uPAR on cell surfaces, uPA localizes plasmin generation, leading to extracellular matrix (ECM) degradation, activation of MMPs and latent growth factors, and facilitating cell invasion and migration, all tightly regulated by PAI-1/2 inhibitors. uPA/uPAR complexes interact with integrins and vitronectin to activate ERK/MAPK, Rac1, and FAK pathways, supporting cell proliferation, survival, and chemotaxis. uPA is physiologically vital for wound healing, angiogenesis, and embryogenesis, while pathologically, it drives cancer metastasis (with high uPA levels indicating poor prognosis), atherosclerosis, and chronic inflammation via ECM remodeling.

Specificity

uPA Antibody [F9E24] detects endogenous levels of total uPA protein.

Background

Urokinase-type plasminogen activator (uPA, PLAU) is a 411-residue secreted serine protease of the chymotrypsin family essential for pericellular proteolysis in the plasminogen activation system. Synthesized as a single-chain proenzyme (scuPA, 52 kDa) with an N-terminal growth factor domain (GFD) for uPAR binding, a kringle domain (KD) mediating fibrin and plasminogen interactions, a connecting peptide, and a catalytic serine protease domain (SPD) containing the His204-Asp256-Ser344 triad, uPA is activated by plasmin cleavage to a two-chain form (tc-uPA). By binding uPAR on cell surfaces, uPA localizes plasmin generation, leading to extracellular matrix (ECM) degradation, activation of MMPs and latent growth factors, and facilitating cell invasion and migration, all tightly regulated by PAI-1/2 inhibitors. uPA/uPAR complexes interact with integrins and vitronectin to activate ERK/MAPK, Rac1, and FAK pathways, supporting cell proliferation, survival, and chemotaxis. uPA is physiologically vital for wound healing, angiogenesis, and embryogenesis, while pathologically, it drives cancer metastasis (with high uPA levels indicating poor prognosis), atherosclerosis, and chronic inflammation via ECM remodeling.

使用情報

Application

WB, IF

Dilution

WB	IF
1:1000 - 1:5000	1:50 - 1:100

Reactivity

Human

Source

Rabbit Monoclonal Antibody

MW

48 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

プロトコール

References

https://pubmed.ncbi.nlm.nih.gov/19273310/
https://pubmed.ncbi.nlm.nih.gov/17118695/

uPA Antibody [F9E24]

生物学的記述

使用情報

References

Application Data