Uric Acid

製品コードS3955 バッチS395501

印刷

化学情報

Chemical Structure Synonyms 2,6,8-Trioxypurine, 2,6,8-Trihydroxypurine, 2,6,8-Trioxopurine Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C5H4N4O3
分子量 168.11 CAS No. 69-93-2
Solubility (25°C)* 体外 0.5MNAOH 3.02 mg/mL (17.96 mM)
DMSO 0.01 mg/mL (0.05 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Uric Acid, a normal component of urine, is a product of the metabolic breakdown of purine nucleotides.
in vitro Uric acid activates NFκB in a variety of cell culture models including proximal tubular cells. Uric acid suppresses 1-α hydroxylase mRNA and protein expression in dose dependent and time dependent manner[1].
in vivo Uric acid is synthesized mainly in the liver, intestines and the vascular endothelium as the end product of an exogenous pool of purines, and endogenously from damaged, dying and dead cells, whereby nucleic acids, adenine and guanine, are degraded into uric acid. Uric acid is a strong reactive oxygen species (ROS) and peroxynitrite scavenger and antioxidant. Uric acid may exert fundamental roles in tissue healing via initiating the inflammatory process that is necessary for tissue repair, scavenging oxygen free radicals, and mobilizing progenitor endothelial cells[2].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 HK2 cells
濃度 2.5, 5.0, 7.5, and 10 mg/dL
反応時間 1, 2, 4, 8, 16, and 24 hours
実験の流れ Cells are cultured in Keratinocyte-SFM basal media, supplemented with Bovine Pituitary Extract (20-30 μg/mL) and recombinant Epidermal Growth Factor (0.1-0.2 ng/mL), 5% Fetal Bovine Serum, 100 U/ml penicillin, and 10 g/ml streptomycin. Cells are cultured at 37°C in 95% air-5% CO2 until they were 90% confluent, and then allowed to differentiate for 5-7 days. After serum starvation for 24 hours, cells are stimulated with uric acid. For the dose response experiments, uric acid is given at 2.5, 5.0, 7.5, and 10 mg/dL and the cells are collected after 24 hours of treatment. Uric acid is used at 10 mg/dL for the time course experiments and the cells are collected at: 1, 2, 4, 8, 16, and 24 hours. To prevent uric acid crystal formation in the media, uric acid is dissolved in prewarmed media, and kept at 37°C for a minimum of 30 minutes prior to application in cell culture.

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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