VPA (Valproic acid)

製品コードS3944 バッチS394403

印刷

化学情報

Chemical Structure Synonyms 2-Propylvaleric Acid, Valproate Storage
(From the date of receipt)		 2 years  -20°C  liquid
化学式

C8H16O2
分子量 144.21 CAS No. 99-66-1
Solubility (25°C)* 体外 DMSO 100 mg/mL (693.43 mM)
Ethanol 100 mg/mL (693.43 mM)
Water 2 mg/mL (13.86 mM)
体内 （毎回新しく調製した物を用意してください）
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 VPA (Valproic acid) is a fatty acid with anticonvulsant properties used in the treatment of epilepsy. It is also a histone deacetylase (HDAC) inhibitor and is under investigation for treatment of HIV and various cancers. Valproic acid (VPA) induces autophagy and mitophagy by upregulation of BNIP3 and mitochondrial biogenesis by upregulating PGC-1α. Valproic acid activates Notch-1 signaling.
in vitro Valproic acid (VPA), like lithium, activates Wnt-dependent gene expression, but unlike lithium, it does not inhibit GSK-3β in vivo. This compound can inhibit GSK-3β-mediated phosphorylation of a CREB peptide in vitro. It may activate Wnt-dependent gene expression through inhibition of HDAC, which in turn leads to both increased expression of β-catenin and de-repression of Tcf/Lef (as well as activation of other HDAC-regulated genes). In vitro, VPA can stimulate glutamic acid decarboxylase, which is involved in GABA biosynthesis, and inhibit GABA transaminase, succinic semialdehyde dehydrogenase, and α-ketoglutarate dehydrogenase, enzymes involved in GABA degradation[1]. It relieves HDAC-dependent transcriptional repression and causes hyperacetylation of histones in cultured cells and in vivo. This compound induces differentiation and/or apoptosis of carcinoma cells, PML-RAR-transformed hematopoietic progenitor cells and leukemic blasts from AML patients[2]. In addition to selectively inhibiting the catalytic activity of class I HDACs, it also induces proteasomal degradation of HDAC2[3].
in vivo Valproic acid (VPA) increases the level of the inhibitory neurotransmitter γ-aminobutyric acid (GABA), with acute administration causing a 15-45% increase in GABA in the brains of rodents[1]. It also inhibits tumor growth and metastasis in animal experiments. This compound is a well-tolerated drug even during long-term treatment[2].
密度 0.9 g/mL at 25 °C

プロトコル(参考用のみ)

細胞アッセイ 細胞株 Neuro2A cells
濃度 0.5-5 mM
反応時間 24 h
実験の流れ

Neuro2A cells are treated with VPA (Valproic acid) (0.5-5 mM) or with TSA (300 nm) for 24 h and then histones are isolated. Histone acetylation is assessed by immunoblotting with an antibody specific to acetylated histone H4.
動物実験 動物モデル Rats
投薬量 1.25 mM/kg
投与方法 i.p.

参考

  • https://pubmed.ncbi.nlm.nih.gov/11473107/
  • https://pubmed.ncbi.nlm.nih.gov/11742974/
  • https://pubmed.ncbi.nlm.nih.gov/12840003/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

SLC7A11 is an unconventional H+ transporter in lysosomes [ Cell, 2025, S0092-8674(25)00406-4] PubMed: 40280132
Matrix directs trophoblast differentiation in a bioprinted organoid model of early placental development [ Nat Commun, 2025, 16(1):8267] PubMed: 40940337
A scalable platform for EPSC-Induced MSC extracellular vesicles with therapeutic potential [ Stem Cell Res Ther, 2025, 16(1):426] PubMed: 40765003
Ginsenoside Rg1 Restores Sirt2/Foxo1 Expression and Alleviates Autism-Like Behaviors in a Valproic Acid Induced Male Mouse Model [ Kaohsiung J Med Sci, 2025, e70078.] PubMed: 40622845
Drosophila melanogaster as a rapid in vivo assay system for preclinical anti-seizure medication testing [ Epilepsia Open, 2025, 10(4):1260-1267] PubMed: 40637098
Restoring sweat gland function in mice using regenerative sweat gland cells derived from chemically reprogrammed human epidermal keratinocytes [ Sci Bull (Beijing), 2024, S2095-9273(24)00799-0] PubMed: 39550273
Self-renewing human naïve pluripotent stem cells dedifferentiate in 3D culture and form blastoids spontaneously [ Nat Commun, 2024, 15(1):668] PubMed: 38253551
Inhibition of HDAC activity directly reprograms murine embryonic stem cells to trophoblast stem cells [ Dev Cell, 2024, S1534-5807(24)00326-5] PubMed: 38823394
Inhibition of HDAC activity directly reprograms murine embryonic stem cells to trophoblast stem cells [ Dev Cell, 2024, S1534-5807(24)00326-5] PubMed: 38823394
RNA m6A modification regulates L1 retrotransposons in human spermatogonial stem cell differentiation in vitro and in vivo [ Cell Mol Life Sci, 2024, 81(1):92] PubMed: 38363375

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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