Datasheet

生物学的記述

Specificity	VEGFB Antibody [E11E24] detects endogenous levels of total VEGFB protein.
Background	Vascular Endothelial Growth Factor B (VEGFB) is a homodimeric cytokine belonging to the PDGF/VEGF family that promotes angiogenesis and regulates lipid metabolism in metabolically active tissues such as the heart, skeletal muscle, and brown adipose tissue. VEGFB exists as two isoforms, VEGFB167 (heparin-binding, matrix-associated) and VEGFB186 (secreted form), both featuring a conserved cystine-knot motif with six cysteine residues forming three disulfide bonds that stabilize the characteristic VEGF dimer structure essential for receptor binding. VEGFB binds with high affinity to VEGFR-1 (Flt-1) primarily through specific residues in its receptor-binding loops, activating signaling cascades that support endothelial cell survival and proliferation without strong mitogenic activity compared to VEGF-A. VEGFB enhances vascular permeability and stabilization while uniquely regulating fatty acid transport across endothelial barriers via induction of translocase proteins like CD36 and FABP4/5, thereby coordinating blood vessel formation with tissue energy demands. VEGFB signals through VEGFR-1 homodimers or VEGFR-1/VEGFR-2 heterodimers, activating PI3K/Akt and PLCγ pathways to promote vessel maturation rather than sprouting angiogenesis. Dysregulated VEGFB contributes to cardiovascular pathologies, including ischemic heart disease and atherosclerosis, acting as a therapeutic target for improving cardiac vascularization and metabolic homeostasis.

Specificity

VEGFB Antibody [E11E24] detects endogenous levels of total VEGFB protein.

Background

Vascular Endothelial Growth Factor B (VEGFB) is a homodimeric cytokine belonging to the PDGF/VEGF family that promotes angiogenesis and regulates lipid metabolism in metabolically active tissues such as the heart, skeletal muscle, and brown adipose tissue. VEGFB exists as two isoforms, VEGFB167 (heparin-binding, matrix-associated) and VEGFB186 (secreted form), both featuring a conserved cystine-knot motif with six cysteine residues forming three disulfide bonds that stabilize the characteristic VEGF dimer structure essential for receptor binding. VEGFB binds with high affinity to VEGFR-1 (Flt-1) primarily through specific residues in its receptor-binding loops, activating signaling cascades that support endothelial cell survival and proliferation without strong mitogenic activity compared to VEGF-A. VEGFB enhances vascular permeability and stabilization while uniquely regulating fatty acid transport across endothelial barriers via induction of translocase proteins like CD36 and FABP4/5, thereby coordinating blood vessel formation with tissue energy demands. VEGFB signals through VEGFR-1 homodimers or VEGFR-1/VEGFR-2 heterodimers, activating PI3K/Akt and PLCγ pathways to promote vessel maturation rather than sprouting angiogenesis. Dysregulated VEGFB contributes to cardiovascular pathologies, including ischemic heart disease and atherosclerosis, acting as a therapeutic target for improving cardiac vascularization and metabolic homeostasis.

使用情報

Application

WB

Dilution

WB

1:1000

Reactivity

Rat, Mouse

Source

Rabbit Monoclonal Antibody

MW

22 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

プロトコール

References

https://pubmed.ncbi.nlm.nih.gov/21917115/
https://pubmed.ncbi.nlm.nih.gov/16616187/

Application Data

WB

Validated by Selleck

Lane 1: 293T, Lane 2: 293T (human VEGFB-3×FLAG transfected)