Vinblastine sulfate

製品コードS4505 バッチS450503

印刷

化学情報

Chemical Structure Synonyms NSC-49842, Vincaleukoblastine, 29060-LE Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C46H58N4O9.H2SO4
分子量 909.05 CAS No. 143-67-9
Solubility (25°C)* 体外 DMSO 100 mg/mL (110.0 mM)
Water Insoluble
Ethanol Insoluble
体内 （毎回新しく調製した物を用意してください）
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution
5%DMSO Corn oil

この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。

 1.670mg/ml (1.84mM) Taking the 1 mL working solution as an example, add 50 μL of 33.3 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Vinblastine sulfate inhibits microtubule formation and suppresses nAChR activity with IC50 of 8.9 μM in a cell-free assay, used to treat certain kinds of cancer. Vinblastine sulfate induces autophagy and apoptosis.
in vitro The average terminal half-lives of Vinblastine sulfate is 14.3 h. When incubated in freshly isolated rat hepatocytes, this compound penetrates rapidly and intensely into the cells, probably through a passive diffusion mechanism followed by tight cellular binding[3]. It inhibits the angiogenic response induced by adrenomedullin and is also positive for mitotic slippage, causing micronuclei in mononucleate cells with cytokinesis block[4]. This chemical gives significant increase in micronucleated mononucleated cells at concentrations that produced approximately 50% cell death and cytostasis or less as calculated using RPD, RICC and RCC[2].
in vivo Vinblastine is a widely used anticancer drug with undesired side effects [6]. A combination of this compound and RAP at very low doses against human HCC gets a satisfactory antiangiogenic effect in vivo[4]. The clinically relevant dose of this chemical inhibits palmitoylation of tubulin in vivo in CEM cells (effect on depalmitoylation of tubulin)[5].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 Chinese hamster ovary (CHO) cells
濃度 1% (v/v) (dissolved in DMSO)
反応時間 3h
実験の流れ

Six-well treatment plates are set up that contained 5 × 104 cells/mL in each well, suspended in 3 mL culture medium, and these are treated with this compound for 3 h followed by 21 h growth.

参考

  • https://pubmed.ncbi.nlm.nih.gov/8516349/
  • https://pubmed.ncbi.nlm.nih.gov/19666138/
  • https://pubmed.ncbi.nlm.nih.gov/2088769/
  • https://pubmed.ncbi.nlm.nih.gov/14508526/
  • https://pubmed.ncbi.nlm.nih.gov/17202812/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Induction of cell death by the CXCR2 antagonist SB225002 in colorectal cancer and stromal cells [ Biomed Pharmacother, 2025, 188:118203] PubMed: 40412356
Cucurbitacin B Exhibits Antitumor Effects on Chordoma Cells via Disruption of Brachyury [ Int J Mol Sci, 2025, 26(8)3864] PubMed: 40332566
Aurora B maintains spherical shape of mitotic cells via simultaneously stabilizing myosin II and vimentin [ J Mol Cell Biol, 2025, mjaf023] PubMed: 40795355
Eribulin Induction of Immunogenic Cell Death (ICD): Comparison With Other Cytotoxic Agents and Temporal Relationship of ICD Biomarkers [ Anticancer Res, 2025, 45(1):39-53] PubMed: 39740820
Patient-derived rhabdomyosarcoma cells recapitulate the genetic and transcriptomic landscapes of primary tumors [ iScience, 2024, 27(10):110862] PubMed: 39319271
A BPTF Inhibitor That Interferes with the Multidrug Resistance Pump to Sensitize Murine Triple-Negative Breast Cancer Cells to Chemotherapy [ Int J Mol Sci, 2024, 25(21)11346] PubMed: 39518898
3-nitropyridine analogues as novel microtubule-targeting agents [ PLoS One, 2024, 19(11):e0307153] PubMed: 39509402
A semiconductor 96-microplate platform for electrical-imaging based high-throughput phenotypic screening [ Nat Commun, 2023, 14(1):7576] PubMed: 37990016
Vinblastine resets tumor-associated macrophages toward M1 phenotype and promotes antitumor immune response [ J Immunother Cancer, 2023, 11(8)e007253] PubMed: 37652576
Vinblastine resets tumor-associated macrophages toward M1 phenotype and promotes antitumor immune response [ J Immunother Cancer, 2023, 10.1136/jitc-2023-007253] PubMed: 37652576

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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