Vincristine

製品コードS9555 バッチS955502

印刷

化学情報

Synonyms

Leurocristine, NSC-67574, 22-Oxovincaleukoblastine

Storage
(From the date of receipt)

3 years -20°C powder

化学式

C₄₆H₅₆N₄O₁₀

分子量

824.96

CAS No.

57-22-7

Solubility (25°C)*

体外

DMSO

100 mg/mL (121.21 mM)

Ethanol

100 mg/mL (121.21 mM)

Water

Insoluble

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	5.000mg/ml (6.06mM)	Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to make it clear; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Vincristine (Leurocristine, NSC-67574, 22-Oxovincaleukoblastine) は、抗腫瘍活性を持つ植物 Vinca rosea Linn. から単離された天然アルカロイドです。Vincristine は有糸分裂紡錘体における微小管形成を阻害し、85 nM の Ki で微小管に結合します。
in vitro	This compound, a microtubule-destabilizing agent (MDA), inhibits the formation of microtubules and mitosis of the cancer cell, which is a victim of intratumoural metabolic resistance that has no important relevance with neither CYP3A4 nor CYP3A5.
in vivo	This compound shows the regression of the tumor in zebrafish xenografts.

プロトコル(参考用のみ)

細胞アッセイ	細胞株	HepG2_EV, HepG2_CYP3A4, HepG_CYP3A5, HepG2_CYP2C8 cells
	濃度	1 μM
	反応時間	24, 48 and 72 h
	実験の流れ	HepG2 sublines (100×10⁴ cells/well) are seeded in 12-well plates and cultured until full confluence. Then, the media is removed, and the cells are exposed to 1 μM drug (docetaxel, paclitaxel or vincristine) or 0.01% DMSO (vehicle control). Samples are collected at 24, 48 and 72 h intervals using TRI Reagent, which is followed by RNA isolation. The RNA yield is assessed via a NanoDrop ND-1000 spectrophotometer, while its quality and integrity are verified by agarose gel electrophoresis.
動物実験	動物モデル	Zebrafish of glioma model
	投薬量	10-20 nM
	投与方法	p.o.

参考

https://pubmed.ncbi.nlm.nih.gov/3986806/
https://pubmed.ncbi.nlm.nih.gov/33775687/
https://pubmed.ncbi.nlm.nih.gov/35199829/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

The ESCRT protein CHMP5 promotes T cell leukemia by enabling BRD4-p300-dependent transcription [ Nat Commun, 2025, 16(1):4133]	PubMed: 40319015
A patient-derived T cell lymphoma biorepository uncovers pathogenetic mechanisms and host-related therapeutic vulnerabilities [ Cell Rep Med, 2025, S2666-3791(25)00102-8]	PubMed: 40147445
CDKN1B (p27/kip1) enhances drug-tolerant persister CTCs by restricting polyploidy following mitotic inhibitors [ Proc Natl Acad Sci U S A, 2025, 122(28):e2507203122]	PubMed: 40623195
Heat shock factor 1 promotes proliferation and chemoresistance in diffuse large B-cell lymphoma by enhancing the cell cycle and DNA repair [ Cell Death Dis, 2025, 16(1):533]	PubMed: 40675964
KAT/3BP: A Metabolism-Targeting Agent with Single and Combination Activity in Aggressive B-Cell Lymphomas [ Cancers (Basel), 2025, 17(12)2034]	PubMed: 40563683
IBCar: Potent Orally Bioavailable Methyl N-[5-(3'-Iodobenzoyl)-1H-Benzimidazol-2-yl]Carbamate for Breast Cancer Therapy [ Cancers (Basel), 2025, 17(15)2526]	PubMed: 40805221
Paclitaxel-mediated microtubule stabilization regulates flexor tendon repair in rats [ Bone Joint Res, 2025, 14(8):685-695]	PubMed: 40765402
Colchicine Binding Site Tubulin Inhibitors Impair Vincristine-Resistant Neuroblastoma Cell Function [ Molecules, 2025, 30(10)2186]	PubMed: 40430358
PTPN21 inhibits cell apoptosis of acute lymphoblastic leukemia induced by chemotherapeutic agents via GADD45A and JNK signaling pathway [ PLoS One, 2025, 20(4):e0322273]	PubMed: 40305474
Patient-derived rhabdomyosarcoma cells recapitulate the genetic and transcriptomic landscapes of primary tumors [ iScience, 2024, 27(10):110862]	PubMed: 39319271

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。