Vincristine

製品コードS9555 バッチS955503

印刷

化学情報

Chemical Structure Synonyms Leurocristine, NSC-67574, 22-Oxovincaleukoblastine Storage
(From the date of receipt)		 3 years -20°C powder
化学式

C46H56N4O10
分子量 824.96 CAS No. 57-22-7
Solubility (25°C)* 体外 DMSO 100 mg/mL (121.21 mM)
Water Insoluble
Ethanol Insoluble
体内 （毎回新しく調製した物を用意してください）
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
5.0mg/ml Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
Clear solution
5% DMSO 95% Corn oil
0.8mg/ml Taking the 1 mL working solution as an example, add 50 μL of 16 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Vincristine (Leurocristine, NSC-67574, 22-Oxovincaleukoblastine) is a natural alkaloid isolated from the plant Vinca rosea Linn. with anti-tumor activity. Vincristine inhibits microtubule formation in mitotic spindle and binds to microtubule with Ki of 85 nM.
in vitro

Vincristine, a microtubule-destabilizing agent (MDA), inhibits the formation of microtubules and mitosis of the cancer cell, which is a victim of intratumoural metabolic resistance that has no important relevance with neither CYP3A4 nor CYP3A5.[2]
in vivo

Vincristine shows the regression of the tumor in zebrafish xenografts.[3]

プロトコル(参考用のみ)

細胞アッセイ 細胞株 HepG2_EV, HepG2_CYP3A4, HepG_CYP3A5, HepG2_CYP2C8 cells
濃度 1 μM
反応時間 24, 48 and 72 h
実験の流れ

HepG2 sublines (100×104 cells/well) are seeded in 12-well plates and cultured until full confluence. Then, the media is removed, and the cells are exposed to 1 μM drug (docetaxel, paclitaxel or vincristine) or 0.01% DMSO (vehicle control). Samples are collected at 24, 48 and 72 h intervals using TRI Reagent, which is followed by RNA isolation. The RNA yield is assessed via a NanoDrop ND-1000 spectrophotometer, while its quality and integrity are verified by agarose gel electrophoresis. 
動物実験 動物モデル Zebrafish of glioma model
投薬量 10-20 nM
投与方法 p.o.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

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FGF1 Protects MCF-7 Cells against Taltobulin through Both the MEKs/ERKs and PI3K/AKT Signaling Pathway [ Biomedicines, 2023, 11(7)1856] PubMed: 37509496
Novel Biologically Active N-Substituted Benzimidazole Derived Schiff Bases: Design, Synthesis, and Biological Evaluation [ Molecules, 2023, 28(9)3720] PubMed: 37175129
α-Mangostin Suppresses Melanoma Growth, Migration, and Invasion and Potentiates the Anti-tumor Effect of Chemotherapy [ Int J Med Sci, 2023, 20(9):1220-1234] PubMed: 37575275

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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