Vitamin C

製品コードS3114 バッチS311403

印刷

化学情報

Synonyms

L-Ascorbic acid

Storage
(From the date of receipt)

3 years -20°C powder (seal)

化学式

C₆H₈O₆

分子量

176.12

CAS No.

50-81-7

Solubility (25°C)*

体外

DMSO

35 mg/mL (198.72 mM)

Water

35 mg/mL (198.72 mM)

Ethanol

Insoluble

体内（毎回新しく調製した物を用意してください）

Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	1.75mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 35 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil	0.58mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 11.6 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Vitamin C (L-Ascorbic acid) is a water-soluble vitamin indicated for the prevention and treatment of scurvy.
in vitro	Ascorbate when combined with copper rapidly reduces the viscosity of DNA solutions and has exhibited some carcinostatic effects on transplanted sarcoma 180 tumours in mice. ^[1] Exposure of HL-60 cells to increasing concentrations of H2O2 results in dose-dependent intracellular accumulation of peroxides, which is accompanied by a decrease in intracellular ascorbic acid and an increase in apoptosis. Ascorbic acid loading also provides strong protection from apoptosis associated with serum- and glucose-free culture of HL-60 cells. ^[2] Vitamin C is found to protect GSH-depleted HL-60 cells in a dose-dependent manner. Vitamin C inhibits the formation of ROS in both normal and GSH-depleted cells. ^[3] Ascorbate (1 mM) generates 161 mM H2O2 in Dulbecco's modified Eagle's medium and induces apoptosis in 50% of HL60 cells, whereas in RPMI 1640 only 83 mM H2O2 is produced and no apoptosis is detected. ^[4]
in vivo	Treatment of rats with Vitamin C 10 min prior to the cisplatin inhibits cisplatin-mediated damage. The enhanced serum creatinine levels which are indicative of renal injury shows a significant reduction with the three doses of Vitamin C tested. ^[5]

製品説明

Vitamin C (L-Ascorbic acid) is a water-soluble vitamin indicated for the prevention and treatment of scurvy.

in vitro

Ascorbate when combined with copper rapidly reduces the viscosity of DNA solutions and has exhibited some carcinostatic effects on transplanted sarcoma 180 tumours in mice. ^[1]

Exposure of HL-60 cells to increasing concentrations of H2O2 results in dose-dependent intracellular accumulation of peroxides, which is accompanied by a decrease in intracellular ascorbic acid and an increase in apoptosis. Ascorbic acid loading also provides strong protection from apoptosis associated with serum- and glucose-free culture of HL-60 cells. ^[2]

Vitamin C is found to protect GSH-depleted HL-60 cells in a dose-dependent manner. Vitamin C inhibits the formation of ROS in both normal and GSH-depleted cells. ^[3]

Ascorbate (1 mM) generates 161 mM H2O2 in Dulbecco's modified Eagle's medium and induces apoptosis in 50% of HL60 cells, whereas in RPMI 1640 only 83 mM H2O2 is produced and no apoptosis is detected. ^[4]

in vivo

Treatment of rats with Vitamin C 10 min prior to the cisplatin inhibits cisplatin-mediated damage. The enhanced serum creatinine levels which are indicative of renal injury shows a significant reduction with the three doses of Vitamin C tested. ^[5]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	Fibroblasts
	濃度	100 μM
	反応時間	2 h
	実験の流れ	Cells were treated with indicated concentration of drug for 2 h.
動物実験	動物モデル	Albino rats
	投薬量	60 mg/kg
	投与方法	p.o.

参考

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Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

RNA m6A modification regulates L1 retrotransposons in human spermatogonial stem cell differentiation in vitro and in vivo [ Cell Mol Life Sci, 2024, 81(1):92]	PubMed: 38363375
Combination of Vitamin C and Lenvatinib potentiates antitumor effects in hepatocellular carcinoma cells in vitro [ PeerJ, 2023, 11:e14610]	PubMed: 36718449
Long noncoding RNA Malat1 inhibits Tead3-Nfatc1–mediated osteoclastogenesis to suppress osteoporosis and bone metastasis [ Res Sq, 2023, rs.3.rs-2405644]	PubMed: 36993303
Long noncoding RNA Malat1 inhibits Tead3-Nfatc1-mediated osteoclastogenesis to suppress osteoporosis and bone metastasis [ Res Sq, 2023, rs.3.rs-2405644]	PubMed: 36993303
An organoid model of colorectal circulating tumor cells with stem cell features, hybrid EMT state and distinctive therapy response profile [ J Exp Clin Cancer Res, 2022, 41(1):86]	PubMed: 35260172
Untargeted Metabolomics Showed Accumulation of One-Carbon Metabolites to Facilitate DNA Methylation during Extracellular Matrix Detachment of Cancer Cells [ Metabolites, 2022, 12(3)267]	PubMed: 35323710
Transcription factor-based gene therapy to treat glioblastoma through direct neuronal conversion [ Cancer Biol Med, 2021, j.issn.2095-3941.2020.0499]	PubMed: 33755378
High throughput proteomic and metabolic profiling identified target correction of metabolic abnormalities as a novel therapeutic approach in head and neck paraganglioma [ Transl Oncol, 2021, 14(8):101146]	PubMed: 34118692
TRIM7 suppresses cell invasion and migration through inhibiting HIF-1α accumulation in clear cell renal cell carcinoma [ Cell Biol Int, 2021, 10.1002/cbin.11750]	PubMed: 34936717
HIF-1α-Mediated Mitophagy Determines ZnO Nanoparticle-Induced Human Osteosarcoma Cell Death both In Vitro and In Vivo [ ACS Appl Mater Interfaces, 2020, 12(43):48296-48309]	PubMed: 33054172

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。