Withaferin A (WFA)

製品コードS8587 バッチS858702

印刷

化学情報

Synonyms

Storage
(From the date of receipt)

3 years -20°C powder

化学式

C₂₈H₃₈O₆

分子量

470.60

CAS No.

5119-48-2

Solubility (25°C)*

体外

DMSO

97 mg/mL (206.11 mM)

Ethanol

4 mg/mL (8.49 mM)

Water

Insoluble

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	4.850mg/ml (10.31mM)	Taking the 1 mL working solution as an example, add 50 μL of 97 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	1.210mg/ml (2.57mM)	Taking the 1 mL working solution as an example, add 50 μL of 24.2 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Withaferin A (WFA)は、チオアルキル化感受性レドックスメカニズムを介して、腫瘍壊死因子誘導性のIκBキナーゼβの活性化を阻害することにより、NF-κB活性化を強力に阻害します。Withaferin Aは、抗腫瘍および抗血管新生作用を持つ中間径フィラメント（IF）タンパク質であるビメンチンに結合します。Withaferin Aは、Withania somniferaから単離されたステロイドラクトンです。
in vitro	Withaferin A (WFA) potently inhibits NFκB activation by preventing the tumor necrosis factor-induced activation of IκB kinase β via a thioalkylation-sensitive redox mechanism. It binds to the intermediate filament (IF) protein, vimentin, by covalently modifying its cysteine residue, which is present in the highly conserved α-helical coiled coil 2B domain, and induces vimentin filaments to aggregate in vitro.
in vivo	Withaferin A (WFA) clearly displays anti-inflammatory characteristics in an acute inflammatory mouse model. Its induced inhibition of capillary growth in a mouse model of corneal neovascularization is compromised in vimentin-deficient mice.

プロトコル(参考用のみ)

細胞アッセイ	細胞株	HUVECs, BAECs,MCF-7 breast cancer cell line, MFT-16 cells
	濃度	5 μM, 10 μM
	反応時間	1 h, 2 h, 24 h
	実験の流れ	To assess the apoptosis activity of Withaferin A (WFA) and 12-D WS, embryonic fibroblast vimentindeficient cell lines (Vim^−/−; MFT-16) and wild-type (Vim^+/+; MFT-6) cells are treated with 5 μM of this compound, 5 μM 12-D WS, or an equivalent amount of vehicle (DMSO) for 24 hr in 37℃-5% CO₂ conditions in complete medium. Apoptotic cells are measured with the Vybrant Apoptosis Assay Kit according to the manufacturer's instructions.
動物実験	動物モデル	8-week-old C57BL/6J mice
	投薬量	0.5 mg/mouse
	投与方法	IP

参考

https://pubmed.ncbi.nlm.nih.gov/17150968/
https://pubmed.ncbi.nlm.nih.gov/17584610/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

LncPTEN1, a long non-coding RNA generated from PTEN, suppresses lung cancer metastasis through the regulation of EMT progress [ Noncoding RNA Res, 2025, 14:25-37]	PubMed: 40521243
Withaferin a modulation of microglia autophagy mitigates neuroinflammation and enhances cognitive function in POCD [ Sci Rep, 2024, 14(1):26112]	PubMed: 39478022
Uveal Melanoma Zebrafish Xenograft Models Illustrate the Mutation Status-Dependent Effect of Compound Synergism or Antagonism [ Invest Ophthalmol Vis Sci, 2024, 65(10):26]	PubMed: 39163035
A Gene Co-Expression Network-Based Drug Repositioning Approach Identifies Candidates for Treatment of Hepatocellular Carcinoma [ Cancers (Basel), 2022, 14(6)1573]	PubMed: 35326724
A redox-sensitive iron-sulfur cluster in murine FAM72A controls its ability to degrade the nuclear form of uracil-DNA glycosylase [ DNA Repair (Amst), 2022, 118:103381]	PubMed: 35908367
HIV-1 Proteins gp120 and Tat Promote Epithelial-Mesenchymal Transition and Invasiveness of HPV-Positive and HPV-Negative Neoplastic Genital and Oral Epithelial Cells [ Microbiol Spectr, 2022, 10(6):e0362222.]	PubMed: 36314970
Withaferin A Triggers Apoptosis and DNA Damage in Bladder Cancer J82 Cells through Oxidative Stress [ Antioxidants (Basel), 2021, 10(7)1063]	PubMed: 34209212
The Activin/FLRG pathway associates with poor COVID-19 outcomes in hospitalized patients [ Mol Cell Biol, 2021, MCB0046721]	PubMed: 34723652
Activin A correlates with the worst outcomes in COVID-19 patients, and can be induced by cytokines via the IKK/NF-kappa B pathway [ bioRxiv, 2021, 10.1101/2021.02.04.429815]	PubMed: N/A
Low Concentration of Withaferin a Inhibits Oxidative Stress-Mediated Migration and Invasion in Oral Cancer Cells [ Biomolecules, 2020, 10(5)E777]	PubMed: 32429564

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。