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Synonyms | WA | Storage (From the date of receipt) |
3 years -20°C powder | ||||||||
化学式 | C28H38O6 |
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分子量 | 470.60 | CAS No. | 5119-48-2 | ||||||||
Solubility (25°C)* | 体外 | DMSO | 97 mg/mL (206.11 mM) | ||||||||
Ethanol | 4 mg/mL (8.49 mM) | ||||||||||
Water | Insoluble | ||||||||||
体内 (毎回新しく調製した物を用意してください) |
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* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | Withaferin A (WFA) potently inhibits NF-κB activation by preventing the tumor necrosis factor-induced activation of IκB kinase β via a thioalkylation-sensitive redox mechanism. Withaferin A binds to the intermediate filament (IF) protein, vimentin with antitumor and antiangiogenesis activity. Withaferin A is a steroidal lactone isolated from Withania somnifera. |
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in vitro | Withaferin A potently inhibits NFκB activation by preventing the tumor necrosis factor-induced activation of IκB kinase β via a thioalkylation-sensitive redox mechanism.[1] Withaferin A binds to the intermediate filament (IF) protein, vimentin, by covalently modifying its cysteine residue, which is present in the highly conserved α-helical coiled coil 2B domain. Withaferin A induces vimentin filaments to aggregate in vitro.[2] |
in vivo | Withagerin A clearly displays anti-inflammatory characteristics in an acute inflammatory mouse model.[1] Withagerin A-induced inhibition of capillary growth in a mouse model of corneal neovascularization is compromised in vimentin-deficient mice.[2] |
細胞アッセイ | 細胞株 | HUVECs, BAECs,MCF-7 breast cancer cell line, MFT-16 cells |
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濃度 | 5 μM, 10 μM | |
反応時間 | 1 h, 2 h, 24 h | |
実験の流れ | To assess the apoptosis activity of WFA and 12-D WS, embryonic fibroblast vimentindeficient cell lines (Vim−/−; MFT-16) and wild-type (Vim+/+; MFT-6) cells are treated with 5 μM WFA, 5 μM 12-D WS, or an equivalent amount of vehicle (DMSO) for 24 hr in 37℃-5% CO2 conditions in complete medium. Apoptotic cells are measured with the Vybrant Apoptosis Assay Kit according to the manufacturer's instructions. |
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動物実験 | 動物モデル | 8-week-old C57BL/6J mice |
投薬量 | 0.5 mg/mouse | |
投与方法 | IP |
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A Gene Co-Expression Network-Based Drug Repositioning Approach Identifies Candidates for Treatment of Hepatocellular Carcinoma [ Cancers (Basel), 2022, 14(6)1573] | PubMed: 35326724 |
A redox-sensitive iron-sulfur cluster in murine FAM72A controls its ability to degrade the nuclear form of uracil-DNA glycosylase [ DNA Repair (Amst), 2022, 118:103381] | PubMed: 35908367 |
HIV-1 Proteins gp120 and Tat Promote Epithelial-Mesenchymal Transition and Invasiveness of HPV-Positive and HPV-Negative Neoplastic Genital and Oral Epithelial Cells [ Microbiol Spectr, 2022, 10(6):e0362222.] | PubMed: 36314970 |
Withaferin A Triggers Apoptosis and DNA Damage in Bladder Cancer J82 Cells through Oxidative Stress [ Antioxidants (Basel), 2021, 10(7)1063] | PubMed: 34209212 |
The Activin/FLRG pathway associates with poor COVID-19 outcomes in hospitalized patients [ Mol Cell Biol, 2021, MCB0046721] | PubMed: 34723652 |
Activin A correlates with the worst outcomes in COVID-19 patients, and can be induced by cytokines via the IKK/NF-kappa B pathway [ bioRxiv, 2021, 10.1101/2021.02.04.429815] | PubMed: N/A |
Low Concentration of Withaferin a Inhibits Oxidative Stress-Mediated Migration and Invasion in Oral Cancer Cells [ Biomolecules, 2020, 10(5)E777] | PubMed: 32429564 |
Withaferin A Induces Oxidative Stress-Mediated Apoptosis and DNA Damage in Oral Cancer Cells [ Front Physiol, 2017, 8:634] | PubMed: 28936177 |
Synergistic inhibition of glioma cell proliferation by Withaferin A and tumor treating fields [ J Neurooncol, 2017, 134(2):259-268] | PubMed: 28681243 |
AshwaMAX and Withaferin A inhibits gliomas in cellular and murine orthotopic models [ J Neurooncol, 2016, 126(2):253-64] | PubMed: 26650066 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。