Wogonin

製品コードS4743 バッチS474301

印刷

化学情報

Chemical Structure Synonyms Vogonin Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C16H12O5
分子量 284.26 CAS No. 632-85-9
Solubility (25°C)* 体外 DMSO 56 mg/mL (197.0 mM)
Ethanol 8 mg/mL (28.14 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Wogonin (Vogonin), a natural and biologically-active flavonoid found in plants, is an inhibitor of CDK9 and does not inhibit CDK2, CDK4 and CDK6 at doses that inhibit CDK9 activity; Also inhibits N-acetyltransferase.
in vitro Wogonin inhibits PMA-induced COX-2 gene expression by inhibiting c-Jun expression and AP-1 activation in A549 cells[1]. Wogonin is an inhibitor of cyclin-dependent kinase 9 (CDK9) and block phosphorylation of the carboxy-terminal domain of RNA polymerase II at Ser. Thus, it reduces RNA synthesis and subsequently rapid downregulation of the short-lived anti-apoptotic protein myeloid cell leukemia 1 (Mcl-1) resulting in apoptosis induction in cancer cells. Wogonin directly binds to CDK9, presumably to the ATP-binding pocketa and does not inhibit CDK2, CDK4 and CDK6 at doses that inhibit CDK9 activity. Wogonin preferentially inhibits CDK9 in malignant compared with normal lymphocytes. Wogonin is a also potent anti-oxidant capable to scavenge •O2− [2]. Wogonin significantly inhibits the translocation of NFATc1 from the cytoplasm to the nucleus and its transcriptional activation activity. It also significantly inhibits osteoclast differentiation and decreases the transcription of osteoclast‑associated immunoglobulin‑like receptor, tartrate‑resistant acid phosphatase and calcitonin receptor[4]. Wogonin Inhibits N-acetyltransferase Activity[5].
in vivo Wogonin suppresses growth of human cancer xenografts in vivo. At doses lethal to tumor cells, wogonin shows no or little toxicity for normal cells and had also no obvious toxicity in animals[2]. Wogonin could induce apoptosis in murine sarcoma S180 thereby inhibiting the tumor growth both in vitro and in vivo[3]. An intraperitoneal injection of 200 mg/kg Wogonin could completely inhibit leukemia and CEM cells[4].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 Human lung carcinoma A549 cells
濃度 100 μM
反応時間 6 h
実験の流れ A549 cells are culture in 24-well plate (1.2×105 cells/well) 1 day before wogonin treatment. DMSO or wogonin is added into A549 cells 1 h before PMA stimulation, and cells are incubated for another 6 h. Cells are collected by trypsin treatment and cell numbers are counted by using a hemocytometer and trypan blue exclusion method.
動物実験 動物モデル ICR species mice
投薬量 10, 20, 40 mg/kg
投与方法 i.v.

カスタマーフィードバック

Data from [Data independently produced by , , Exp Ther Med, 2015, 10(3):1066-1070]

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Identification of EZH2 as Cancer Stem Cell Marker in Clear Cell Renal Cell Carcinoma and the Anti-Tumor Effect of Epigallocatechin-3-Gallate -EGCG [ Cancers -Basel, 2022, 14-174200] PubMed: 36077742
STIM1 Regulates Endothelial Calcium Overload and Cytokine Upregulation During Sepsis [ J Surg Res, 2021, 263:236-244] PubMed: 33713955
Wogonin inhibits osteoclast differentiation by inhibiting NFATc1 translocation into the nucleus. [Geng X, et al. Exp Ther Med, 2015, 10(3):1066-1070] PubMed: 26622440

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。