WST-8

製品コードS7764 バッチS776410

印刷

化学情報

Chemical Structure Synonyms Storage
(From the date of receipt)		 3 years -20°C(in the dark) powder
化学式

C20H13N6NaO11S2
分子量 600.47 CAS No. 193149-74-5
Solubility (25°C)* 体外 Water 100 mg/mL (166.53 mM)
DMSO 30 mg/mL (49.96 mM)
Ethanol Insoluble
体内 （毎回新しく調製した物を用意してください）
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 WST-8 is a water-soluble tetrazolium salt used for assessing cell metabolic activity that produces corresponding formazan dye that absorbs at 460 nm. WST-8 is typically used as a cell viability indicator in cell proliferation assays.
in vitro

WST-8 is reduced by NADH in good yields at neutral pHs in the presence of 1-methoxy PMS to produce the corresponding formazan dye that absorbs at 460 nm. The formazan is soluble to water at concentrations higher than 0.1 M. This compound is useful as a sensitive chromogenic indicator for NADH. It is also applicable to cell proliferation assays as a cell viability indicator.[1]

プロトコル(参考用のみ)

細胞アッセイ 細胞株 Hela cells
濃度 0.5 mM
反応時間 2 h
実験の流れ

A hundred μl of HeLa cell culture in the logarithmic phase is inoculated onto a 96-well microtiter plate, and the plate pre-incubated for 24 h in a CO2 incubator at 37℃. A 10 μl of the working solution containing WST-8 and lmethoxy PMS (0.5 mM and 20 μM, respectively, as the final concentration) is added to each well; it is incubated for an additional 2 h. The absorbance of each well is measured at 450 nm with a reference wavelength at 650 nm with an M-UVmax microplate reader.

参考

  • https://pubmed.ncbi.nlm.nih.gov/18966866/

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長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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