WZ811

製品コードS2912 バッチS291201

印刷

化学情報

Chemical Structure Synonyms N/A Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

 

C18H18N4
 
分子量 290.36 CAS No. 55778-02-4
Solubility (25°C)* 体外 DMSO 30 mg/mL (103.32 mM)
Water Insoluble
Ethanol Insoluble
体内 （毎回新しく調製した物を用意してください）
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O

この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。

 1.500mg/ml (5.17mM) Taking the 1 mL working solution as an example, add 50 μL of 30 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 WZ811 is a highly potent competitive CXCR4 antagonist with EC50 of 0.3 nM.
in vitro WZ811 is a highly potent competitive antagonist of CXCR4. This compound is effective in counteracting SDF-1 on cAMP reduction at doses as low as a few nanomoles. It blocks SDF-1-mediated invasion with EC50 of 5.2 nM. [1]

プロトコル(参考用のみ)

キナーゼアッセイ Binding affinity
For compound screening based on a competition binding assay, 2 × 104 MDA-MB-231 cells in 200μL of medium are seeded in an 8-well slide chamber 2 days before the experiments. Various concentrations of this compound (1, 10, 100, and 1000 nM) are added to the separate wells and incubated for 10 min at room temperature, and then the cells are fixed in 4% ice-cold paraformaldehyde. The cells are rehydrated in phosphate-buffered saline (PBS) and blocked to eliminate nonspecific binding. The slides are subsequently incubated for 45 min at room temperature with 0.05 μg/mL biotinylated 3, washed three times with PBS, and incubated in streptavidin-rhodamine (1:150 dilution) for 30 min at room temperature. Finally, the slides are washed with PBS and mounted in an antifade mounting solution.

参考

  • https://pubmed.ncbi.nlm.nih.gov/17958344/

カスタマーフィードバック

, , Invest New Drugs, 2017, 35(6):718-732

Data independently produced by , , Dr.Milica Pesic from Institute for Biological Research

Data independently produced by , , Dr. Milica Pesic from Institute for Biological Research

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

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Exploring the Interplay of RUNX2 and CXCR4 in Melanoma Progression [ Cells, 2024, 13(5)408] PubMed: 38474372
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The CXCR4-CXCL12 axis promotes T-cell reconstitution via efficient hematopoietic immigration [ J Genet Genomics, 2022, S1673-8527(22)00125-4] PubMed: 35483564
Single-cell transcriptomic reveals molecular diversity and developmental heterogeneity of human stem cell-derived oligodendrocyte lineage cells [ Nat Commun, 2021, 12(1):652] PubMed: 33510160
TGFβR-SMAD3 Signaling Induces Resistance to PARP Inhibitors in the Bone Marrow Microenvironment [ Cell Rep, 2020, 33(1):108221] PubMed: 33027668

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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