Datasheet

生物学的記述

Specificity	XPF Antibody [D21K23] detects endogenous levels of total XPF protein.
Background	Excision repair cross‑complementing 4 (ERCC4, XPF) is a structure‑specific endonuclease that forms a heterodimeric complex with ERCC1, serving as a core nuclease in multiple DNA‑repair pathways, including nucleotide excision repair (NER), interstrand crosslink repair (ICLR), and certain double‑strand break (DSB)‑repair contexts. XPF contains an endonuclease domain and a helix‑helicase‑like domain that positions the ERCC1–XPF complex at DNA junctions, where it cleaves 3ʹ‑flap structures and excises the 5ʹ side of DNA lesions in cooperation with the 3ʹ endonuclease XPG during NER dual‑incision events. In NER, the ERCC1–XPF complex is recruited to damaged DNA by the XPA protein and participates in the removal of bulky, helix‑distorting lesions such as those induced by UV radiation and chemotherapeutic crosslinking agents, whereas in ICLR it is targeted to psoralen‑ and other chemically induced interstrand crosslinks through interactions with FANCD2 and the FA core complex, linking XPF activity to the Fanconi‑anemia DNA‑repair network. In DSB‑repair contexts such as single‑strand annealing, XPF and ERCC1 execute incisions at resected DNA junctions to remove non‑homologous tails and facilitate repair‑template annealing, and XPF has also been shown to persist at DNA lesions longer in repair‑deficient backgrounds.

Specificity

XPF Antibody [D21K23] detects endogenous levels of total XPF protein.

Background

Excision repair cross‑complementing 4 (ERCC4, XPF) is a structure‑specific endonuclease that forms a heterodimeric complex with ERCC1, serving as a core nuclease in multiple DNA‑repair pathways, including nucleotide excision repair (NER), interstrand crosslink repair (ICLR), and certain double‑strand break (DSB)‑repair contexts. XPF contains an endonuclease domain and a helix‑helicase‑like domain that positions the ERCC1–XPF complex at DNA junctions, where it cleaves 3ʹ‑flap structures and excises the 5ʹ side of DNA lesions in cooperation with the 3ʹ endonuclease XPG during NER dual‑incision events. In NER, the ERCC1–XPF complex is recruited to damaged DNA by the XPA protein and participates in the removal of bulky, helix‑distorting lesions such as those induced by UV radiation and chemotherapeutic crosslinking agents, whereas in ICLR it is targeted to psoralen‑ and other chemically induced interstrand crosslinks through interactions with FANCD2 and the FA core complex, linking XPF activity to the Fanconi‑anemia DNA‑repair network. In DSB‑repair contexts such as single‑strand annealing, XPF and ERCC1 execute incisions at resected DNA junctions to remove non‑homologous tails and facilitate repair‑template annealing, and XPF has also been shown to persist at DNA lesions longer in repair‑deficient backgrounds.

使用情報

Application

WB

Dilution

WB

1:1000

Reactivity

Human, Rat, Monkey

Source

Rabbit Monoclonal Antibody

MW

104 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

プロトコール

References

https://pubmed.ncbi.nlm.nih.gov/30563071/
https://pubmed.ncbi.nlm.nih.gov/30165384/

Application Data

WB

Validated by Selleck

Lane 1: Hela, Lane 2: HT-1080, Lane 3: 293T