Y-27632

製品コードS6390 バッチS639001

印刷

化学情報

Chemical Structure Synonyms N/A Storage
(From the date of receipt)		 3 years -20°C powder
化学式

C14H21N3O
分子量 247.34 CAS No. 146986-50-7
Solubility (25°C)* 体外 DMSO 49 mg/mL (198.1 mM)
Ethanol 49 mg/mL (198.1 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Y-27632 is a selective ROCK1 and ROCK2 inhibitor with a Ki of 140 nM and 300nM in a cell-free assay, exhibits >200-fold selectivity over other kinases, including PKC, cAMP-dependent protein kinase, MLCK and PAK.
in vitro

Y-27632 primes human induced pluripotent stem cells (hIPSCs) to selectively differentiate towards mesendodermal lineage via epithelial-mesenchymal transition-like modulation.[2]
in vivo

Y27632, a specific inhibitor of ROCK, supresses ROCK as well as altered pathways and leads to a significant enhancement in corneal nerve regeneration.[3]

プロトコル(参考用のみ)

細胞アッセイ 細胞株 Human iPSC line nciPS02, RC01001-B, Female; Pluripotent stem cells
濃度 20 µM
反応時間 --
実験の流れ

The hiPSC line is cultured on six-well plates coated with 1% Matrigel. Pluripotent stem cells are maintained in mTeSR1 (Stem Cell) medium supplemented with 100 µg/mL Normocin coated with 1% Matrigel. For differentiation, hiPSC colonies are detached from the culture dish using Accutase Cell Dissociation Reagent. Then, cells are collected as a single-cell suspension in Essential 8 Flex medium containing 20 µm Y27632. Cells are distributed at a density of 3500 cells in 100 µL of medium per well into 96-well U-bottom plates. After 24 h of incubation at 37 ℃, fresh E8 medium without Y27632 is added to a total volume of 200 µL per well, and the plates are incubated at 37℃ continuously. Next, cell aggregates are cultured in E6-based differentiation medium containing 2% Matrigel, 10 µm SB431542, 4 ng mL 1 basic FGF, and 2.5 ng mL 1 BMP4. On day 3 of differentiation, 200 ng mL 1 LDN-193189 and 50 g mL 1 FGF are added to induce cranial neural crest cell formation. On day 12, all cell aggregates are transferred to 24-well low-attachment plates in 500 µL of organoid maturation mediumcontaining 1% Matrigel to induce self-assembly of the epidermis. Half of the medium is replaced after 16 d after differentiation. On day 17, the organoids are collected for the subsequent experiments.
動物実験 動物モデル C57BL/6J mice
投薬量 5 nM
投与方法 eye drops

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Ancestral allele of DNA polymerase gamma modifies antiviral tolerance [ Nature, 2024, 10.1038/s41586-024-07260-z] PubMed: 38570685
Osteopontin drives neuroinflammation and cell loss in MAPT-N279K frontotemporal dementia patient neurons [ Cell Stem Cell, 2024, S1934-5909(24)00094-8] PubMed: 38626772
Bioelectric stimulation controls tissue shape and size [ Nat Commun, 2024, 15(1):2938] PubMed: 38580690
Nongenetic Optical Modulation of Pluripotent Stem Cells Derived Cardiomyocytes Function in the Red Spectral Range [ Adv Sci (Weinh), 2024, 11(3):e2304303] PubMed: 37948328
An adapted protocol to derive microglia from stem cells and its application in the study of CSF1R-related disorders [ Mol Neurodegener, 2024, 19(1):31] PubMed: 38576039
Targeting EMSY-mediated methionine metabolism is a potential therapeutic strategy for triple-negative breast cancer [ Cell Rep Med, 2024, 5(2):101396] PubMed: 38290515
Auranofin repurposing for lung and pancreatic cancer: low CA12 expression as a marker of sensitivity in patient-derived organoids, with potentiated efficacy by AKT inhibition [ J Exp Clin Cancer Res, 2024, 43(1):88] PubMed: 38515178
Biosafe cerium oxide nanozymes protect human pluripotent stem cells and cardiomyocytes from oxidative stress [ J Nanobiotechnology, 2024, 22(1):132] PubMed: 38532378
Genetic and functional correction of argininosuccinate lyase deficiency using CRISPR adenine base editors [ Am J Hum Genet, 2024, 111(4):714-728] PubMed: 38579669
Cell mechanics regulate the migration and invasion of hepatocellular carcinoma cells via JNK signaling [ Acta Biomater, 2024, S1742-7061(24)00036-9] PubMed: 38272199

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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