Datasheet

生物学的記述

Specificity	YY1 Antibody [M11B11] detects endogenous levels of total YY1 protein.
Background	Yin Yang 1 (YY1) is a ubiquitously expressed zinc‑finger transcription factor that combines classical DNA binding with architectural chromatin functions to regulate a broad spectrum of genes involved in embryogenesis, differentiation, replication, and proliferation. YY1 contains four Cys‑Cys‑His‑His‑type zinc fingers that recognize specific GC‑rich motifs in promoters and enhancers, and its N‑ and C‑terminal domains facilitate recruitment of coactivators such as histone acetyltransferases and corepressors such as histone deacetylases, enabling context‑dependent activation or repression of target genes like CREB, c‑myc, p53, and histone H4. YY1 helps orchestrate three‑dimensional genome organization by promoting enhancer–promoter chromatin loops within topologically associating domains, linking distal regulatory elements to transcription initiation sites and thereby stabilizing lineage‑specific and developmental transcription programs. YY1 is essential for implantation‑stage embryos and for the expression of pluripotency factors such as Oct4 and Sox2, and its conditional ablation in neural crest and other progenitor populations leads to midembryonic lethality and severe structural defects, underscoring its role in cell‑fate decisions and tissue morphogenesis. YY1 frequently shows elevated expression and contributes to tumor growth, survival, and metastasis by modulating stem‑like properties, cell‑cycle regulators, and apoptotic pathways, and its dysregulation has been associated with poor clinical outcomes across multiple carcinomas.

Specificity

YY1 Antibody [M11B11] detects endogenous levels of total YY1 protein.

Background

Yin Yang 1 (YY1) is a ubiquitously expressed zinc‑finger transcription factor that combines classical DNA binding with architectural chromatin functions to regulate a broad spectrum of genes involved in embryogenesis, differentiation, replication, and proliferation. YY1 contains four Cys‑Cys‑His‑His‑type zinc fingers that recognize specific GC‑rich motifs in promoters and enhancers, and its N‑ and C‑terminal domains facilitate recruitment of coactivators such as histone acetyltransferases and corepressors such as histone deacetylases, enabling context‑dependent activation or repression of target genes like CREB, c‑myc, p53, and histone H4. YY1 helps orchestrate three‑dimensional genome organization by promoting enhancer–promoter chromatin loops within topologically associating domains, linking distal regulatory elements to transcription initiation sites and thereby stabilizing lineage‑specific and developmental transcription programs. YY1 is essential for implantation‑stage embryos and for the expression of pluripotency factors such as Oct4 and Sox2, and its conditional ablation in neural crest and other progenitor populations leads to midembryonic lethality and severe structural defects, underscoring its role in cell‑fate decisions and tissue morphogenesis. YY1 frequently shows elevated expression and contributes to tumor growth, survival, and metastasis by modulating stem‑like properties, cell‑cycle regulators, and apoptotic pathways, and its dysregulation has been associated with poor clinical outcomes across multiple carcinomas.

使用情報

Application

WB, IP, IF, FCM, ChIP

Dilution

WB	IP	IF	FCM	CHIP
1:1000	1:200	1:800	1:100	1:50

Reactivity

Human, Mouse, Rat, Monkey

Source

Rabbit Monoclonal Antibody

MW

65 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

プロトコール

References

https://pubmed.ncbi.nlm.nih.gov/16314846/
https://pubmed.ncbi.nlm.nih.gov/33102493/

Application Data

WB

Validated by Selleck

Lane 1: Hela, Lane 2: MCF7, Lane 3: C2C12, Lane 4: COS-7