Z-FA-FMK

製品コードS7391 バッチS739101

化学情報

	Synonyms	(1S)-Z-FA-FMK	Storage (From the date of receipt)	3 years -20°C powder 1 years -80°C in solvent
	化学式	C₂₁ H₂₃ F N₂ O₄
	分子量	386.42	CAS No.	197855-65-5
Solubility (25°C)*	体外	DMSO	77 mg/mL (199.26 mM)
		Ethanol	34 mg/mL warmed with 50ºC water bath (87.98 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Z-FA-FMK ((1S)-Z-FA-FMK) is an irreversible cysteine protease inhibitor, and also inhibits effector caspases.
in vitro	Z-FA-FMK inhibits the degradation of fibrillar collagen by fibroblasts and osteoclasts. ^[2] Z-FA-FMK inhibits LPS-induced cytokine production via inhibition of NF-kappaB-dependent gene expression in macrophages. ^[3] Z-FA-FMK efficiently blocks T cell proliferation induced by mitogens and IL-2 in vitro. ^[4]
in vivo	Z-FA-FMK significantly increases pneumococcal growth in both lungs and blood in a mouse model of intranasal pneumococcal infection. ^[4] Z-FA-FMK blocks reovirus infection of Ras oncogenic tumours and host heart tissues in severe combined immunodeficiency mice. ^[5]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	PBMCs
	濃度	~100 μM
	反応時間	72 hours
	実験の流れ	T cell proliferation following mitogen stimulation is determined using [³H]thymidine incorporation. In brief, PBMCs or purified T cells are seeded in a 96-well plate and stimulated with either PHA (5 μg/ml), costimulated with anti-CD3 mAb (5 μg/ml) and anti-CD28 mAb (2.5 μg/ml) or PMA plus ionomycin in the presence or absence of z-FA-FMK. The cells are cultured for 72 h with the last 16 h pulsed with [methyl-³H]thymidine (0.037 MBq). The cells are harvested onto glass fiber filter mats using a Tomtec automated multiwell harvester.
動物実験	動物モデル	Mouse model of pneumococcal disease
	投薬量	~25 mg/kg
	投与方法	i.v.

参考

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Pharmacological targeting of TFIIH suppresses KRAS mutant pancreatic ductal adenocarcinoma and synergizes with TRAIL [ Cancer Res, 2022, can.21.4222]	PubMed: 35819261
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Targeting multiple cell death pathways extends the shelf life and preserves the function of human and mouse neutrophils for transfusion [ Sci Transl Med, 2021, 13(604)eabb1069]	PubMed: 34321317
Design of modular autoproteolytic gene switches responsive to anti-coronavirus drug candidates [ Nat Commun, 2021, 12(1):6786]	PubMed: 34811361
SARS-CoV-2 requires cholesterol for viral entry and pathological syncytia formation [ Elife, 2021, 10e65962]	PubMed: 33890572
Development of a Cell-Based Luciferase Complementation Assay for Identification of SARS-CoV-2 3CLpro Inhibitors [ Viruses, 2021, 13(2)173]	PubMed: 33498923
Autophagy Participates in Lysosomal Vacuolation-Mediated Cell Death in RGNNV-Infected Cells [ Front Microbiol, 2020, 30;11:790]	PubMed: 32425913
Ferroptosis Is Involved in Alcohol-Induced Cell Death in vivo and in vitro [ Biosci Biotechnol Biochem, 2020, 18;1-8]	PubMed: 32419644
[ Oncogene, 2018, ]	PubMed: 29326439
The Tumor Suppressor p53 Limits Ferroptosis by Blocking DPP4 Activity [ Cell Rep, 2017, 20(7):1692-1704]	PubMed: 28813679

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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