Z-FA-FMK

製品コードS7391 バッチS739102

印刷

化学情報

Synonyms

(1S)-Z-FA-FMK

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₂₁ H₂₃ F N₂ O₄

分子量

386.42

CAS No.

197855-65-5

Solubility (25°C)*

体外

DMSO

77 mg/mL (199.26 mM)

Ethanol (warmed with 50ºC water bath)

48 mg/mL (124.21 mM)

Water

Insoluble

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension

CMC-NA

≥5mg/ml

Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Z-FA-FMK ((1S)-Z-FA-FMK) is an irreversible cysteine protease inhibitor, and also inhibits effector caspases.
in vitro	Z-FA-FMK inhibits the degradation of fibrillar collagen by fibroblasts and osteoclasts. ^[2] This compound inhibits LPS-induced cytokine production via inhibition of NF-kappaB-dependent gene expression in macrophages. ^[3] It efficiently blocks T cell proliferation induced by mitogens and IL-2 in vitro. ^[4]
in vivo	Z-FA-FMK significantly increases pneumococcal growth in both lungs and blood in a mouse model of intranasal pneumococcal infection. ^[4] This compound blocks reovirus infection of Ras oncogenic tumours and host heart tissues in severe combined immunodeficiency mice. ^[5]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	PBMCs
	濃度	~100 μM
	反応時間	72 hours
	実験の流れ	T cell proliferation following mitogen stimulation is determined using [³H]thymidine incorporation. In brief, PBMCs or purified T cells are seeded in a 96-well plate and stimulated with either PHA (5 μg/ml), costimulated with anti-CD3 mAb (5 μg/ml) and anti-CD28 mAb (2.5 μg/ml) or PMA plus ionomycin in the presence or absence of z-FA-FMK. The cells are cultured for 72 h with the last 16 h pulsed with [methyl-³H]thymidine (0.037 MBq). The cells are harvested onto glass fiber filter mats using a Tomtec automated multiwell harvester.
動物実験	動物モデル	Mouse model of pneumococcal disease
	投薬量	~25 mg/kg
	投与方法	i.v.

参考

https://pubmed.ncbi.nlm.nih.gov/4073501/
https://pubmed.ncbi.nlm.nih.gov/2069557/
https://pubmed.ncbi.nlm.nih.gov/11290751/

https://pubmed.ncbi.nlm.nih.gov/16951345/
https://pubmed.ncbi.nlm.nih.gov/20834102/

+ 展开

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Fluorescence-guided tumor resection with a cathepsin B-activatable, EGFR-targeted probe and a dual-mode surgical exoscope [ Eur J Med Chem, 2025, 300:118108]	PubMed: 40911969
Pharmacological targeting of TFIIH suppresses KRAS mutant pancreatic ductal adenocarcinoma and synergizes with TRAIL [ Cancer Res, 2022, can.21.4222]	PubMed: 35819261
Potential drug discovery for COVID-19 treatment targeting Cathepsin L using a deep learning-based strategy [ Comput Struct Biotechnol J, 2022, 20:2442-2454]	PubMed: 35602976
Targeting multiple cell death pathways extends the shelf life and preserves the function of human and mouse neutrophils for transfusion [ Sci Transl Med, 2021, 13(604)eabb1069]	PubMed: 34321317
Design of modular autoproteolytic gene switches responsive to anti-coronavirus drug candidates [ Nat Commun, 2021, 12(1):6786]	PubMed: 34811361
SARS-CoV-2 requires cholesterol for viral entry and pathological syncytia formation [ Elife, 2021, 10e65962]	PubMed: 33890572
Development of a Cell-Based Luciferase Complementation Assay for Identification of SARS-CoV-2 3CLpro Inhibitors [ Viruses, 2021, 13(2)173]	PubMed: 33498923
Autophagy Participates in Lysosomal Vacuolation-Mediated Cell Death in RGNNV-Infected Cells [ Front Microbiol, 2020, 30;11:790]	PubMed: 32425913
Ferroptosis Is Involved in Alcohol-Induced Cell Death in vivo and in vitro [ Biosci Biotechnol Biochem, 2020, 18;1-8]	PubMed: 32419644
[ Oncogene, 2018, ]	PubMed: 29326439

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。