Z-LEHD-FMK TFA

製品コードS7313 バッチS731303

印刷

化学情報

Chemical Structure Synonyms Caspase-9 Inhibitor Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C34H44F4N6O12
分子量 804.74 CAS No. 524746-03-0
Solubility (25°C)* 体外 Water 100 mg/mL (124.26 mM)
 
 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Z-LEHD-FMK TFA (Caspase-9 Inhibitor) is a specific, irreversible Caspase-9 inhibitor.
in vitro

Z-LEHD-FMK is a cell-permeable, competitive and irreversible inhibitor of enzyme caspase-9, which helps in cell survival[2].
in vivo

Z-LEHD-FMK TFA (Caspase-9 Inhibitor) is a cell-permeable, competitive and irreversible inhibitor of enzyme caspase-9.

プロトコル(参考用のみ)

細胞アッセイ 細胞株 HCT116 and SW480 cells
濃度 20 μM
反応時間 2 h
実験の流れ

HCT116 and SW480 cells (as indicated) were pretreated with the inhibitors Z-IETD-FMK (caspase 8 inhibitor), Z-LEHD-FMK (caspase 9 inhibitor), Z-VDAVD-FMK (caspase 2 inhibitor), and Z-AEVD-FMK (caspase 10 inhibitor; 20 μm each) for 2 h, after which TRAIL (50 ng/ml) was added. After 4 h (or 16 h for caspase 3 activation) of TRAIL exposure, extracts were collected and analyzed by Western blotting for the expression of the different proteins.
動物実験 動物モデル Male Wistar albino rats
投薬量 0.8 μM/kg
投与方法 i.v.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

ER stress induces caspase-2-tBID-GSDME-dependent cell death in neurons lytically infected with herpes simplex virus type 2 [ EMBO J, 2023, e113118.] PubMed: 37646198
AMIGO2 attenuates innate cisplatin sensitivity by suppression of GSDME-conferred pyroptosis in non-small cell lung cancer [ J Cell Mol Med, 2023, 27(16):2412-2423] PubMed: 37438979
AMIGO2 attenuates innate cisplatin sensitivity by suppression of GSDME-conferred pyroptosis in non-small cell lung cancer [ J Cell Mol Med, 2023, 27(16):2412-2423] PubMed: 37438979
Zika virus causes placental pyroptosis and associated adverse fetal outcomes by activating GSDME [ Elife, 2022, 11e73792] PubMed: 35972780
Autophagy promotes GSDME-mediated pyroptosis via intrinsic and extrinsic apoptotic pathways in cobalt chloride-induced hypoxia reoxygenation-acute kidney injury [ Ecotoxicol Environ Saf, 2022, 242:113881] PubMed: 35863214
Physapruin A Enhances DNA Damage and Inhibits DNA Repair to Suppress Oral Cancer Cell Proliferation [ Int J Mol Sci, 2022, 23(16)8839] PubMed: 36012104
RhFGF21 Protects Epidermal Cells against UVB-Induced Apoptosis through Activating AMPK-Mediated Autophagy [ Int J Mol Sci, 2022, 23(20)12466] PubMed: 36293323
HCA -2-Hydroxy-Docosahexaenoic Acid Induces Apoptosis and Endoplasmic Reticulum Stress in Pancreatic Cancer Cells [ Int J Mol Sci, 2022, 23-179902] PubMed: 36077299
Aconitine induces cell apoptosis via mitochondria and death receptor signaling pathways in hippocampus cell line [ Res Vet Sci, 2022, 143:124-133] PubMed: 35026629
Identification of ferroptosis as a novel mechanism for antitumor activity of natural product derivative a2 in gastric cancer [ Acta Pharm Sin B, 2021, 11(6):1513-1525] PubMed: 34221865

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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